狂犬病、犬瘟热和犬细小病毒病三联灭活疫苗免疫效果评价  

Evaluation of the Efficacy of Inactivated Triplex Vaccine of Rabies-Canine Distemper-Canine Parvovirus

作　　者：龚成燕 陈杰[3] 潘虹军 罗国良[1] 刘梦佳 胡博[1] 赵建军 GONG Chengyan;CHEN Jie;PAN Hongjun;LUO Guoliang;LIU Mengjia;HU Bo;ZHAO Jianjun(Key Laboratory of Special Animal Epidemic Disease of Ministry of Agricultural rural affairs,Institute of Special Economic Animal and Plant Sciences,CAAS,Changchun 130112,China;College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University,Daqing 163319,China;College of Veterinary Medicine,Northwest A&F University,Yangling 712100,China;College of Traditional Chinese Medicine,Jilin Agricultural University,Changchun 130118,China;Jinan Customs District,Jinan 250200,China)

机构地区：[1]中国农业科学院特产研究所农业农村部经济动物疫病重点实验室,长春130112 [2]黑龙江八一农垦大学动物科技学院,大庆163319 [3]西北农林科技大学,杨凌712100 [4]吉林农业大学中药材学院,长春130112 [5]济南海关,济南250200

出　　处：《中国动物传染病学报》2023年第3期82-90,共9页Chinese Journal of Animal Infectious Diseases

基　　金：国家重点研发计划项目(2017YFD0501600);黑龙江八一农垦大学引进人才科研启动计划(XYB201912);国家自然科学基金(31972714)。

摘　　要：狂犬病、犬瘟热和犬细小病毒病是当前危害养犬业的三种重要传染病。为探讨狂犬病毒(RABV)、犬瘟热病毒(CDV)和犬细小病毒(CPV)灭活制备三联灭活疫苗的可行性,将该3种病毒HEP-Flury株、LN(10)1株和JL(18)1-Beagle株分别在BHK-21、Vero/DogSLAM(VDS)和F81细胞培养,经β-丙内酯灭活剂灭活后,分别与三种不同类型免疫佐剂(MONTANIDE GEL 02、ADJ-801(W)、Al(OH)3)配制成三联灭活疫苗。疫苗经三次免疫比格犬后,通过测定动物血清抗体水平和攻毒保护情况评价其免疫效果。实验结果显示,不同佐剂的三联灭活疫苗对增强比格犬RABV、CDV和CPV血清抗体应答反应效果不同。其中ADJ-801(W)佐剂能显著提高针对RABV、CDV和CPV三种病毒的抗体水平,疫苗三次免疫比格犬后血清抗体效价分别为7.4 EU/mL、1∶50和1∶1024;而Al(OH)3佐剂效果较差,三免后抗体效价分别为4.01 EU/mL、1∶6和1∶256。疫苗三次免疫后,分别应用强毒CDV SD(14)7株和CPVJL(18)1-Beagle株对比格犬进行攻毒实验,实验结果显示,ADJ-801(W)组对SD(14)7和JL(18)1-Beagle攻毒保护率均为100%,MONTANIDE GEL 02组攻毒保护率均为60%,而Al(OH)3组攻毒保护率均为0,表明ADJ-801(W)佐剂制备的狂犬病、犬瘟热和犬细小病毒病三联灭活疫苗对比格犬提供较好的免疫保护作用。本研究为犬狂犬病、犬瘟热和犬细小病毒病三联灭活疫苗的研制奠定了基础。Rabies,canine distemper and canine parvovirus disease are three important contagious diseases,threatening the dog-raising industry.To evaluate the immunogenicity of inactivated triplex Rabies virus(RABV),Canine distemper virus(CDV),Canine parvovirus(CPV)emulsified with three adjuvants MONTANIDE GEL 02,ADJ-801(W)and Al(OH)3,the HEP-Flury,LN(10)1 and JL(18)1-Beagle strains of these viruses were cultured on BHK-21,Vero/DogSLAM(VDS)and F81 cells and inactivated byβ-propiolactone,respectively.Then inactivated viruses were mixed and emulsified with each of adjuvants MONTANIDE GEL 02,ADJ-801(W)and Al(OH)3.Beagles were immunized with different triplex inactivated vaccines three times and challenged afterwards.Blood samples were collected at different times.The results demonstrated that the inactivated triplex vaccines with different adjuvants had varying effects on enhancing the antibody titers of CDV,CPV and RABV.The vaccine with ADJ-801(W)adjuvant significantly induced the serum antibody responses and the antibody titers of CDV,CPV and RABV were 7.4 EU/mL,1∶50 and 1∶1024,respectively.In contrast,adjuvant Al(OH)3 showed poor enhancement as the antibody titers of CDV,CPV and RABV were 4.01 EU/mL,1∶6 and 1∶256,respectively.Subsequently,beagles were challenged with virulent CDV(SD(14)7)or CPV(JL(18)1-Beagle)strain.The inactivated triplex vaccines with ADJ-801(W)provided 100%protection while the vaccine with MONTANIDE GEL 02 produced 60%protection.The vaccine with Al(OH)3 had no protection.In conclusion,this study laid a foundation for the development of an inactivated triplex vaccine against rabies-canine distemper-canine parvovirus disease.

关 键 词：狂犬病病毒 犬瘟热病毒 犬细小病毒 灭活疫苗 佐剂 

分 类 号：S858.292[农业科学—临床兽医学]