Flavonoids in safflower extract reduce cisplatin-induced damage to human follicle dermal papilla cells by inhibiting DNA damage and Rad17/Chk1/Cdc25C signaling  

作　　者：FU-MING TSAI PING-HSUN LU LU-KAI WANG CHAN-YEN KUO MAO-LIANG CHEN CHUN-HUA WANG 

机构地区：[1]Department of Research,Taipei Tzu Chi Hospital,Buddhist Tzu Chi Medical Foundation,New Taipei City,231,Taiwan [2]Department of Chinese Medicine,Taipei Tzu Chi Hospital,Buddhist Tzu Chi Medical Foundation,New Taipei City,231,Taiwan [3]School of Post-Baccalaureate Chinese Medicine,Tzu Chi University,Hualien,970,Taiwan [4]Department of Life Sciences,National Science and Technology Council,Taipei,106,Taiwan [5]Department of Dermatology,Taipei Tzu Chi Hospital,Buddhist Tzu Chi Medical Foundation,New Taipei City,231,Taiwan [6]School of Medicine,Tzu Chi University,Hualien,970,Taiwan

出　　处：《BIOCELL》2023年第8期1793-1802,共10页生物细胞（英文）

基　　金：supported by the Taipei Tzu Chi Hospital through grants from the Buddhist Tzu Chi Medical Foundation under the Numbers TCRD-TPE-110-13 and TCRD-TPE-111-23,Taipei,Taiwan.

摘　　要：Background:Cisplatin is a chemotherapeutic agent commonly used clinically for the treatment of various human cancers.Patients often reduce the use of cisplatin due to its side effects,which in turn affects its treatment.This study explored the mechanism of action of safflower extract as an adjuvant traditional Chinese medicine for chemotherapy.Methods:Primary human follicle dermal papilla cells(HFDPCs)were used as target cells for cisplatininduced damage to hair cells.Western blotting was used to investigate the molecular targets of cisplatin and safflower extract in causing HFDPCs damage.Cell survival and cell cycle were analyzed by mitochondrial staining reagent WST-1 and propidium iodide.Results:Cisplatin could reduce the viability of HFDPCs without causing cell death.Cisplatin increased the level of phospho-Rad17 in HFDPCs and activated the Chk1/Cdc25C signaling to reduce the expression of Cdc2 protein,thereby arresting the cells in the G2/M phase.The combination of safflower extract and the flavonoids could effectively inhibit the signal transduction of Rad17/Chk1/Cdc25 in cisplatin-treated cells and reduce the cell population in the G2/M phase.Finally,we also confirmed that safflower extract could effectively inhibit the damage to HFDPCs caused by cisplatin,mainly at the level of reducing the DNA damage caused by cisplatin.Conclusions:Safflower extract can be used as an adjuvant Chinese medicine for chemotherapy to reduce the damage caused by chemotherapy to normal hair follicle cells.

关 键 词：Safflower extract CISPLATIN Human hair follicle dermal papilla cells Rad17 Hair loss Cell cycle 

分 类 号：R73[医药卫生—肿瘤]