A YAP/TAZ-CD54 axis is required for CXCR2-CD44-tumor-specific neutrophils to suppress gastric cancer  

作　　者：Pingping Nie Weihong Zhang Yan Meng Moubin Lin Fenghua Guo Hui Zhang Zhenzhu Tong Meng Wang Fan Chen Liwei An Yang Tang Yi Han Ruixian Yu Wenjia Wang Yuanzhi Xu Linxin Wei Zhaocai Zhou Shi Jiao 

机构地区：[1]CAS Center for Excellence in Molecular Cell Science,Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,University of Chinese Academy of Sciences,Shanghai 200031,China [2]State Key Laboratory of Genetic Engineering,School of Life Sciences,Zhongshan Hospital,Fudan University,Shanghai 200438,China [3]Tongji University Cancer Center,Department of Medical Ultrasound,Shanghai Tenth People's Hospital,Ultrasound Research and Education Institute,Tongji University School of Medicine,Shanghai200072,China [4]Postdoctoral Station of Clinical Medicine,Shanghai Tenth People's Hospital,Tongji University School of Medicine,Shanghai 200072,China [5]Tumor Immunology and Gene Therapy Center,Shanghai Eastem Hepatobiliary Surgery Hospital,Shanghai 200438,China [6]Department of General Surgery,Yangpu Hospital,Tongji University School of Medicine,Shanghai 200090,China [7]Department of General Surgery,Hua'shan Hospital,Fudan University Shanghai Medical College,Shanghai 200040,China [8]Department of Stomatology,Shanghai Tenth People's Hospital,Tongji University School of Medicine,Shanghai 200072,China [9]Collaborative Innovation Center for Cancer Personalized Medicine,School of Public Health,Nanjing Medical University,Nanjing 211166,China

出　　处：《Protein & Cell》2023年第7期515-533,共19页蛋白质与细胞（英文版）

基　　金：supported by the National Key R&D Program ofChina(Nos.2020YFA0803200,2017YFA0504504,and 2018YFA0107500);National Natural Science Foundation of China Grants(Nos.82003014,82150112,81725014,92168116,31930026,81972599,82173276,81822035);the Key Program for Basic Research of Shanghai(No.19JC1415600);Shanghai Rising-Star Program(Nos.22QA1407200,22QA1407300);Open Research Fund of State Key Laboratory of Genetic Engineering,Fudan University(No.SKLGE2103);the China Postdoctoral Science Foundation(No.2020M671231).

摘　　要：As an important part of tumor microenvironment,neutrophils are poorly understood due to their spatiotemporal heterogeneity in tumorigenesis.Here we defined,at single-cell resolution,CD44-CxCR2-neutrophils as tumor-specific neutrophils(tsNeus)in both mouse and human gastric cancer(GC).We uncovered a Hippo regulon in neutrophils with unique YAP signature genes(e.g.,ICAM1,CD14,EGR1)distinct from those identified in epithelial and/or cancer cells.Importantly,knockout of YAP/TAz in neutrophils impaired their differentiation into CD54+tsNeus and reduced their antitumor activity,leading to accelerated GC progression.Moreover,the relative amounts of CD54+tsNeus were found to be negatively associated with GC progression and positively associated with patient survival.Interestingly,GC patients receiving neoadjuvant chemotherapy had increased numbers of CD54+tsNeus.Furthermore,pharmacologically enhancing YAP activity selectively activated neutrophils to suppress refractory GC,with no significant inflammation-related side effects.Thus,our work characterized tumor-specific neutrophils in GC and revealed an essential role of YAP/TAZ-CD54 axis in tsNeus,opening a new possibility to develop neutrophil-based antitumor therapeutics.

关 键 词：NEUTROPHILS YAP TAZ gastriccancer 

分 类 号：R735.2[医药卫生—肿瘤]