三叶青超微粉对酒精性肝损伤大鼠肠道菌群的调节作用  

Effects of Tetrastigma hemsleyanum Superfine Powder on Intestinal Microflora in Rats with Alcohol-Induced Liver Injury

作　　者：程晓阳 廖明[1] 何全光 莫财锋 黄茂康 黄梅华 CHENG Xiaoyang;LIAO Ming;HE Quanguang;MO Caifeng;HUANG Maokang;HUANG Meihua(Guangxi Medical University,Nanning 530021,China;Dinghu People's Hospital,Zhaoqing 526070,China;Institute of Agro-products Processing and Technology,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;Guangxi Huayin Medical Laboratory Center,Nanning 530000,China)

机构地区：[1]广西医科大学,广西南宁530021 [2]肇庆市鼎湖区人民医院,广东肇庆526070 [3]广西壮族自治区农业科学院农产品加工研究所,广西南宁530007 [4]广西华银医学检验所有限公司,广西南宁530000

出　　处：《食品工业科技》2023年第18期415-424,共10页Science and Technology of Food Industry

基　　金：广西农业科学院团队项目(2021YT110);广西高校中青年教师科研能力提升项目(2019KY0127)。

摘　　要：目的:研究三叶青超微粉能否缓解酒精引起的肝损伤,并探讨其保护作用机制,为开发酒精性肝损伤辅助治疗产品提供新的研究思路。方法:连续给予大鼠8周酒精制备肝损伤模型,同时给予大鼠灌胃不同剂量的三叶青超微粉,观察其对酒精性肝损伤的保护作用。检测大鼠血清中ALT、AST、ALP和肝组织中GSH、SOD、MDA的变化,观察肝组织病理切片损伤情况,并采集粪便提取基因组DNA,进行16S rDNA全长测序分析。结果:与模型组比较,三叶青中、高剂量(0.5和1.0 g·kg^(−1))可显著降低大鼠ALT、AST和ALP水平及MDA含量(P<0.05),提高SOD和GSH活性,减轻肝组织病变。肝组织切片证实,三叶青超微粉明显改善酒精所引起的肝细胞肿胀和脂肪变性,治疗效果类似于阳性药联苯双酯。肠道菌群分析表明,酒精性肝损伤大鼠肠道菌群结构发生显著变化;三叶青、阳性药干预对肠道菌群在门、属、种水平上较模型组均有一定程度的改善;三叶青能有效恢复因酒精增加的普雷沃菌属,降低的约氏乳杆菌、毛杆菌丰度,效果优于阳性药对照组(P<0.05)。结论:首次验证了三叶青超微粉对大鼠酒精性肝损伤有明显的保护作用,其机制可能与其抗氧化应激作用及肠道菌群的调节有关。Objective:To investigate and reveal protective mechanism which the Tetrastigma hemsleyanum(SYQ)ultramicro powder alleviate alcohol-induced liver injury,and to provide a new research idea for clinical treatment of alcohol-induced liver injury.Methods:The rat model of alcoholic liver injury was established by continuous administration of alcohol for 8 weeks.At the same time,rats were given different doses SYQ ultramicro powder by intragastric to observe its protective effect on liver injury.Changes of ALT,AST,ALP and GSH,SOD,MDA were detected in serum and liver tissue,respectively.Pathological sections of liver tissue were used to observe the damage.Genomic DNA was extracted from feces and analyzed by 16S rDNA full-length sequencing.Results:Compared with the model group,medium and high doses of SYQ(0.5 and 1.0 g·g^(−1))significantly(P<0.05)reduced the levels of ALT,AST ALP and MDA,increased the activities of SOD and GSH,and alleviated the liver lesions in rats.The pathological sections revealed that SYQ ultramicro powder dramatically alleviated liver cells swelling and steatosis,and these effectiveness were similar to the group of bifendate.Intestinal microflora analysis showed that the intestinal microflora structure significantly changed in rats with alcoholic liver injury.Compared with the model group,both SYQ and bifendate intervention improved the intestinal flora to a certain extent at phylum,genus and species level.SYQ effectively recovered the abundance of Prevotella,Lactobacillus_johnsonii,Raoultibacter_massiliensis altered by alcohol,and the effect of medium doses of SYQ was better than positive control group(P<0.05).Conclusion:The SYQ superfine powder had a significant protective effect on alcoholic-induced liver injury in rats,the mechanism may be related to its antioxidant stress and intestinal flora regulation.

关 键 词：三叶青 超微粉 酒精性肝损伤 氧化应激 肠道菌群 

分 类 号：TS201.4[轻工技术与工程—食品科学]