功能红曲米中的吡啶类色素化合物  

Pyridine pigments from functional Monascus rice

作　　者：刘冰语 郑晓明 刘安安 徐飞 魏茜 胡友财 LIU Bing-yu;ZHENG Xiao-ming;LIU An-an;XU Fei;WEI Qian;HU You-cai(Key Laboratory of Bioactive Substance and Function of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050,China)

机构地区：[1]中国医学科学院、北京协和医学院药物研究所,天然药物活性物质与功能国家重点实验室,北京100050

出　　处：《药学学报》2023年第8期2442-2447,共6页Acta Pharmaceutica Sinica

基　　金：国家杰出青年科学基金资助项目(82225042)。

摘　　要：对功能性红曲米中的微量化学成分进行研究,以探索潜在的生物活性物质。运用MCI柱色谱、Sephadex LH-20凝胶柱色谱、制备液相色谱等多种色谱方法,对其乙酸乙酯提取物部分进行分离,应用质谱(mass spectrometry,MS)、红外(infrared radiation,IR)、核磁共振(nuclear magnetic resonance,NMR)等波谱学方法对分离得到的天然产物进行鉴定。从中分离并鉴定了两个新颖的吡啶类红曲红色素成分,分别为monascopyridine G(1)和monascopyridine H(2)。通过CDOCKER分子对接实验探究其过氧化物酶体增殖物激活受体γ(peroxisome proliferators-activated receptor-gamma,PPARγ)激动活性,发现其与受体蛋白有较好的结合力。并进一步对该类化合物的生物合成途径进行了推测,为选择性生产该类潜在活性物质提供参考。The trace chemical components in functional Monascus rice were studied to explore the potential bioactive substances.MCI column,Sephadex LH-20 gel,and preparative liquid chromatography were used to purified the ethyl acetate extract from functional Monascus rice.Two novel pyridine Monascus pigments were isolated and identified,named monascopyridine G(1)and monascopyridine H(2),respectively based on extensive mass spectrometry(MS),infrared radiation(IR),and nuclear magnetic resonance(NMR)analysis.The molecular docking experiments between compounds 1 and 2 and peroxisome proliferators-activated receptor-gamma(PPARγ)showed that they exhibited obvious binding force with the receptor protein.Besides,the biosynthetic pathways of the two compounds were proposed,which provide a valuable reference for the selective production of these potential bioactive substances.

关 键 词：红曲米 色素 分子对接 过氧化物酶体增殖物激活受体Γ激动剂 生物合成 

分 类 号：R931[医药卫生—生药学]