气相色谱-离子迁移谱法同时检测婴儿食品中3种香兰素类化合物  

Simultaneous determination of 3 kinds of vanillin compounds in infant foods by gas chromatography-ion mobility spectrometry

作　　者：魏宇 胡文艳 刘文 李文山 敬国兴[1] 于建娜 刘文杰 WEI Yu;HU Wen-Yan;LIU Wen;LI Wen-Shan;JING Guo-Xing;YU Jian-Na;LIU Wen-Jie(College of Chemical Engineering,Xiangtan University,Xiangtan 411105,China)

机构地区：[1]湘潭大学化工学院,湘潭411105

出　　处：《食品安全质量检测学报》2023年第14期219-226,共8页Journal of Food Safety and Quality

基　　金：国家自然科学基金项目(21864021);湖南省教育厅重点项目(21A0091)。

摘　　要：目的建立气相色谱-离子迁移谱法同时测定婴儿食品中香兰素、甲基香兰素和乙基香兰素含量的方法。方法样品经盐酸酸化、乙腈提取、离心后,采用Agilent J&W HP-5色谱柱(30 m×530 mm,1.5μm)进行分离,在电晕放电离子迁移谱的正模式下进行谱图采集,以迁移时间和保留时间定性,标准曲线外标法定量。结果3种香兰素类化合物在0.2~20.0μg/mL范围内呈现良好的线性关系,相关系数在0.9988~0.9998,方法定量限在0.08~0.12mg/kg,加标回收率为75.32%~105.56%,相对标准偏差为1.95%~8.31%(n=6)。结论该方法灵敏、准确,适用于同时检测婴儿食品中香兰素、甲基香兰素和乙基香兰素的含量。Objective To establish a method for simultaneous determination of vanillin,methyl vanillin and ethyl vanillin content in infant foods by gas chromatography-ion mobility spectrometry.Methods The samples were acidified with hydrochloric acid,extracted with acetonitrile,and centrifuged.The target compounds were separated by an HP-5 column(30 m×530 mm,1.5μm)after samples extraction.With the positive mode of the corona discharge ion mobility spectrometry,the drift time and retention time were qualitatively determined,and the external standard was used for calibration.Results Vanillin,methyl vanillin,and ethyl vanillin showed a good linear relationship in the concentration range of 0.2-20.0μg/mL,the correlation coefficients were 0.9988-0.9998,the limits of quantitation were 0.08-0.12 mg/kg,and the recovery rates were 75.32%-105.56%,with the relative standard deviations of 1.95%-8.31%(n=6).Conclusion This method is sensitive,accurate and suitable for simultaneous determination of vanillin,methyl vanillin and ethyl vanillin in infant foods.

关 键 词：气相色谱与离子迁移谱法 同时检测 香兰素 婴儿食品 

分 类 号：O657[理学—分析化学] TS207.3[理学—化学]