Elucidating cellular interactome of chikungunya virus identifies host dependency factors  

作　　者：Peiqi Yin Xia Jian Yihan Liu Yuwen Liu Lu Lv Haoran Cui Leiliang Zhang 

机构地区：[1]Department of Clinical Laboratory Medicine,The First Affiliated Hospital of Shandong First Medical University&Shandong Provincial Qianfoshan Hospital,Jinan,250013,China [2]NHC Key Laboratory of Systems Biology of Pathogens,Institute of Pathogen Biology,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,100176,China [3]Department of Pathogen Biology,School of Clinical and Basic Medical Sciences,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan,250117,China [4]Medical Science and Technology Innovation Center,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan,250117,China

出　　处：《Virologica Sinica》2023年第4期497-507,共11页中国病毒学（英文版）

基　　金：supported by National Natural Science Foundation of China (82072270 and 82272306);Taishan Scholars Program (tstp20221142);Shandong Provincial Natural Science Foundation (ZR2021QC095);Academic Promotion Programme of Shandong First Medical University (2019LJ001).

摘　　要：Chikungunya virus(CHIKV)is a re-emerging mosquito-transmitted RNA virus causing joint and muscle pain.To better understand how CHIKV rewires the host cell and usurps host cell functions,we generated a systematic CHIKV-human protein-protein interaction map and revealed several novel connections that will inform further mechanistic studies.One of these novel interactions,between the viral protein E1 and STIP1 homology and U-box containing protein 1(STUB1),was found to mediate ubiquitination of E1 and degrade E1 through the proteasome.Capsid associated with G3BP1,G3BP2 and AAAþATPase valosin-containing protein(VCP).Furthermore,VCP inhibitors blocked CHIKV infection,suggesting VCP could serve as a therapeutic target.Further work is required to fully understand the functional consequences of these interactions.Given that CHIKV proteins are conserved across alphaviruses,many virus-host protein-protein interactions identified in this study might also exist in other alphaviruses.Construction of interactome of CHIKV provides the basis for further studying the function of alphavirus biology.

关 键 词：Chikungunya virus(CHIKV) INTERACTOME STIP1 homology and U-box containing protein 1(STUB1) Valosin-containing protein(VCP) CAPSID 

分 类 号：R373[医药卫生—病原生物学]