一株产纤维素酶菌株的分离纯化及其产酶性质研究  

Isolation and purification of a cellulose-producing strain and its enzyme-producing properties

作　　者：黄翠欣 张桂容 刘军 舒林 曹荣 温雪瓶 李丽 HUANG Cuixin;ZHANG Guirong;LIU Jun;SHU Lin;CAO Rong;WEN Xueping;LI Li(College of Bioengineering,Sichuan University of Science&Engineering,Yibin 644000,China;Sichuan Slan Biotechnology Co.Ltd.,Chengdu 610000,China;Sichuan Taiyuanjing Vinegar Co.Ltd.,Zigong 643000,China;Soild-state Fermentation Resource Utilization Key Laboratory of Sichuan Province,Yibin 644000,China)

机构地区：[1]四川轻化工大学生物工程学院,四川宜宾644000 [2]四川思来生物科技有限公司,四川成都610000 [3]四川太源井醋业有限公司,四川自贡643000 [4]固态发酵资源利用四川省重点实验室,四川宜宾644000

出　　处：《食品与发酵工业》2023年第17期37-43,共7页Food and Fermentation Industries

基　　金：四川省科技厅重点研发项目(2021YFN0095);固态发酵资源利用四川省重点实验室开放基金项目(2021GTJC05)。

摘　　要：该研究以四川晒醋醋醅为研究对象,从醋醅中筛选出1株纤维素酶活力较高的霉菌,结合生理生化特性和ITS基因序列同源性分析对菌株进行鉴定,通过单因素试验、正交试验探究其产酶条件及酶学性质。结果显示,筛选出的霉菌为横梗霉菌(Lichtheimia ramosa),在菌悬液浓度为3.5×10^(6)CFU/mL、1%接种量、30℃和180 r/min恒温振荡培养24 h的条件下提取粗酶液,并置于50℃反应30 min;其内切葡聚糖酶活力在pH 5.0时达到最大值为95.92 U/mL,β-葡萄糖苷酶活力在pH 6.0时达到最大值为47.57 U/mL。该研究揭示了横梗霉菌在醋醅发酵过程中产生了内切葡聚糖酶和β-葡萄糖苷酶,且二者较好的pH值稳定性和热稳定性,研究结果为四川晒醋的生产提供了一定的参考价值。In this research,a fungus with high cellulase activity was screened from Cupei of Sichuan sun vinegar.Its physiological-biochemical characteristics were studied and analyzed by ITS sequence.The conditions of enzyme producing and enzymatic properties of the strain were investigated by single factor and orthogonal experiments.The strain was identified as Lichtheimia ramosa,the crude enzyme solution was extracted under the following conditions:3.5×10^(6) CFU/mL of bacterial suspension,1% of inoculation volume,30℃,cultured for 24 h at 180 r/min,and the reaction was performed at 50℃ for 30 min.The max activities of endoglucanase and β-glucosidase were 95.92 U/mL and 47.57 U/mL at pH 5.0 and 6.0.This study revealed that endoglucanase and β-glucosidase were produced by L.ramosa during the fermentation of Cupei,and had well stability of pH and heat stress,which provided some reference value for the production of Sichuan sun vinegar.

关 键 词：四川晒醋 分离鉴定 横梗霉菌 内切葡聚糖酶 Β-葡萄糖苷酶 

分 类 号：TS264.22[轻工技术与工程—发酵工程]