2种消毒剂对牙周超声洁牙机独立水路消毒效果的比较  

Comparison of the effect of two disinfectants on the independent waterway disinfection of a periodontal ultrasonic scaler

作　　者：侯雅蓉 倪佳 周俏怡 高钰琳[1] 张小燕 陈晓川 HOU Yarong;NI Jia;ZHOU Qiaoyi;GAO Yulin;ZHANG Xiaoyan;CHEN Xiaochuan(School of Nursing,Southern Medical University,Guangzhou 510515,China;Stomatological Hospital,School of Stomatology,Southern Medical University,Guangzhou 510280,China)

机构地区：[1]南方医科大学护理学院,广东广州510515 [2]南方医科大学口腔医院(口腔医学院),广东广州510280

出　　处：《口腔疾病防治》2023年第12期855-862,共8页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：广东省基础与应用基础研究基金项目(区域联合基金⁃青年基金项目)(2021A1515110872)。

摘　　要：目的 比较体积分数3%过氧化氢溶液和500 mg/L含氯消毒液分别用于牙周超声洁牙机独立水路的消毒效果,为口腔科临床水路消毒管理工作提供参考。方法 将牙周科18台超声洁牙机随机分成3组,1个对照组和2个实验组,每组6台。对照组更换无菌水源和供水管,对机器及手柄连接线内不可更换的水路管道不做处理;实验组更换无菌水源和供水管,对不可更换的水路管道采用消毒剂消毒处理。实验1组采用3%过氧化氢溶液消毒,实验2组采用500 mg/L的含氯消毒液消毒。3组均在当天消毒前、消毒后即刻、消毒后1~10 d每天开诊前在洁牙机工作端出水口取水检测,进行水样细菌培养及菌落计数,比较组内及组间消毒前后菌落数,水中菌落数不超过100 CFU/mL为合格。消毒后在每组菌落数首次出现超标时进行一次细菌种类质谱鉴定分析。在消毒后第10天时扫描电镜观察3组水路管道内壁生物膜情况。结果 消毒前,3组细菌菌落计数均不合格,差异无统计学意义(F=2.549,P=0.111)。消毒后,各时间点对照组洁牙机出水口水样细菌菌落数均超标,培养出瓜类鞘氨醇单胞菌、哈特草螺菌、皮氏罗尔斯通氏菌3种细菌。实验1组消毒后第1、2天的水样菌落数达标,与对照组相比,差异有统计学意义(P<0.05),消毒后第3天起菌落数超标,同时培养出鞘氨醇单胞菌、哈特草螺菌、皮氏罗尔斯通氏菌3种细菌。实验2组消毒后第1~6天菌落数达标,与对照组比较,差异有统计学意义(P<0.05),消毒后第7天起每日菌落数超标,同时培养出哈特草螺菌和皮氏罗尔斯通氏菌2种细菌。实验2组消毒后的10 d平均菌落数少于实验1组(P<0.001)。电镜下见2个实验组生物膜厚度明显低于对照组。结论 牙周超声洁牙机独立水路存在污染;3%过氧化氢溶液和500 mg/L含氯消毒液两种消毒剂对洁牙机内水路均有消毒作用,500 mg/L含氯消毒液消毒效果优�Objective To compare the disinfection effect of 3%(v/v)hydrogen peroxide and 500 mg/L chlorinecontaining disinfectants in the independent waterway of a periodontal ultrasonic scaler to provide a reference for clinical waterway disinfection management in stomatology departments.Methods The 18 ultrasonic scalers were randomly divided into 3 groups of 6 units:the control group,experimental group 1(3% hydrogen peroxide disinfectant group),and experimental group 2(500 mg/L chlorinecontaining disinfectant group).The replaceable parts of the independent waterway pipes of the 3 groups of ultrasonic scalers were replaced,and the water supply was supplied with sterile distilled water(DW).In the control group,special treatment was not applied to the nonreplaceable pipe part.In experimental group 1,the 3% hydrogen peroxide was used to disinfect nonreplaceable pipelines.In experimental group 2,the nonreplaceable part was disinfected with the 500 mg/L chlorinecontaining disinfectant.The water sample was taken from the outlet of the scaler working part in the three groups for monitoring before disinfection,immediately after disinfection and 10 consecutive days after disinfection.Bacteria in the water samples were cultured for the colony counts.Then,the bacterial culture data were compared between groups.The qualified criterion of the water sample was that the number of bacterial colonies was less than or equal to 100 CFU/mL.After disinfection,a bacterial species mass spectrometry identification analysis was carried out when the number of bacterial colonies in each group exceeded the standard for the first time.Biofilms from the inner wall of the tube in the three groups were observed under an electron microscope on the 10th day after disinfection.Results There were no significant differences between the three groups before disinfection(F=2.549,P=0.111).The number of bacterial colonies in the spout of 6 scalers in the control group all exceeded the standard,and three kinds of bacteria were cultured:Sphingomonas melonis,Herbaspir

关 键 词：超声波洁牙机 独立水路 内置水管 菌落计数 消毒管理 3%过氧化氢溶液 含氯消毒液 消毒时机 细菌质谱鉴定 生物膜 

分 类 号：R78[医药卫生—口腔医学]