Identification of novel antisense long non-coding RNA APMAP-AS that modulates porcine adipogenic differentiation and inflammatory responses  

作　　者：ZHANG Lin-zhen HE Li WANG Ning AN Jia-hua ZHANG Gen CHAI Jin WU Yu-jie DAI Chang-jiu LI Xiao-han LIAN Ting LI Ming-zhou JIN Long 

机构地区：[1]Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province,Sichuan Agricultural University,Chengdu 611130,P.R.China [2]College of Grassland Science and Technology,Sichuan Agricultural University,Chengdu 611130,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第8期2483-2499,共17页农业科学学报（英文版）

基　　金：supported by grants from the National Key R&D Program of China(2021YFA0805903,2020YFA0509500 and 2021YFD1300800);the Sichuan Science and Technology Program,China(2021ZDZX0008,2021YFYZ0009,2021YFYZ0030 and 2022JDJQ0054).

摘　　要：Long non-coding RNAs(lncRNAs)are emerging as powerful regulators of adipocyte differentiation,fat metabolism and gene expression.However,the functional roles and mechanisms of lncRNAs in these processes remain unclear.Here,we identified a novel antisense transcript,named APMAP-AS,transcribed from adipocyte membrane-associated protein(APMAP)in the pig genome.APMAP-AS and APMAP were highly expressed in retroperitoneal adipose of obese pigs,compared with that in control pigs.Using a bone mesenchymal stem cells(BMSCs)adipogenic differentiation model,we found that APMAP-AS positively regulated adipogenic differentiation.APMAP-AS had the potential to form an RNA–RNA duplex with APMAP,and increased the stability of APMAP mRNA.Additionally,APMAP-AS promoted lipid metabolism and inhibited the expression of inflammatory factors.These findings of a natural antisense transcript for a regulatory gene associated with lipid synthesis might further our understanding of lncRNAs in driving adaptive adipose tissue remodeling and preserving metabolic health.

关 键 词：lncRNA ADIPOGENESIS lipid metabolism INFLAMMATION RNA-RNA duplex 

分 类 号：S852.2[农业科学—基础兽医学]