Transcriptomic and proteomic studies of condylar ossification of the temporomandibular joint in porcine embryos  

作　　者：Lei Xiang Yongfeng Li Xuewen Wang HuaWei Liu Ping Chang Xiaodan Mu Tengyue Tianteng Min Hu 

机构地区：[1]Beijing Research Institute of Traumatology and Orthopaedics,Beijing,China [2]Department of Stomatology,Beijing Friendship Hospital,Capital Medical University,Beijing,China [3]Institute for Laboratory Animal Resources,National Institutes for Food and Drug Control,Beijing,China [4]Department of Stomatology,the First Medical Center of PLA General Hospital,Beijing,China [5]State Key Laboratory of West China College of Stomatology,Sichuan University,Cheng Du,China

出　　处：《Animal Models and Experimental Medicine》2023年第4期294-305,共12页动物模型与实验医学（英文）

基　　金：National Key Research and Development Program,Grant/Award Number:2017YFB1104103;Municipal Health Commission,Grant/Award Number:BJRITO-RDP-2023。

摘　　要：Background:The ossification mechanism of the temporomandibular joint(TMJ)condyle remains unclear in human embryo.The size and structure of TMJ,shape of articular disc and the characteristics of omnivorous chewing in the pig are similar to those of humans.The pig is an ideal animal for studying the mechanism of ossification of the TMJ condyle during the embryonic period.Method:In a previous study by our group,it was found that there was no condylar ossification on embryonic day(E)45,but the ossification of condyle occurred between E75 and E90.In this study,a total of 12 miniature pig embryos on E45 and E85 were used.Six embryos were used for tissue sections(3 in each group).The remaining six embryos were used for transcriptomic and proteomic studies to find differential genes and proteins.The differentially expressed genes in transcriptome and proteomic analysis were verified by QPCR.Results:In total,1592 differential genes comprising 1086 up-regulated genes and 506 down-regulated genes were screened for fold changes of≥2 to≤0.5 between E45 and E85.In the total of 4613 proteins detected by proteomic analysis,there were 419 differential proteins including 313 up-regulated proteins and 106 down-regulated proteins screened for fold changes of≥2 to≤0.5 between E45 and E85.A total of 36 differential genes differing in both transcriptome and proteome analysis were found.QPCR analysis showed that 14 of 15 selected genes were consistent with transcriptome analysis.Conclusion:Condylar transcriptome and proteomic analysis during the development of TMJ in miniature pigs revealed the regulatory genes/proteins of condylar ossification.

关 键 词：CONDYLE EMBRYO miniature pig OSSIFICATION proteomics transcriptome 

分 类 号：R782.6[医药卫生—口腔医学] R-332[医药卫生—临床医学]