基于生物信息学技术分析恶性胸膜间皮瘤信号通路与核心基因  

Bioinformatics-based analysis of signaling pathways and core genes in malignant interstitial pleural dermatomas

作　　者：傅海寿 尤剑彬 谢海花[1] 王军军 Fu Haishou;You Jianbin;Xie Haihua;Wang Junjun(Department of Clinical Laboratory,Fujian Provincial Hospital South Branch,Fuzhou 350008,China;Department of Clinical Laboratory,Fujian Provincial Hospital,Fuzhou 350008,China)

机构地区：[1]福建省立医院南院检验科,福州350008 [2]福建省立医院检验科,福州350000

出　　处：《实用医技杂志》2023年第2期82-86,I0001-I0003,共8页Journal of Practical Medical Techniques

摘　　要：目的利用生物信息学技术分析恶性胸膜间皮瘤(MPM)的潜在作用机制与核心基因。方法从基因表达综合(GEO)数据库中下载GSE51024的基因表达谱。使用R语言包筛选差异表达基因(DEGs),并对DEGs进行基因本体论(gene ontology,GO)及系统分析基因功能、基因组信息数据库(kyoto encyclopedia of genes and genomes,KEGG)富集分析。随后,构建DEGs的蛋白质-蛋白质相互作用(PPI)网络,并筛选出网络中的核心基因。最后,在癌症基因组图谱(TCGA)队列中验证核心基因的总生存期。结果共获得574个DEGs,包括176个上调基因和398个下调基因。此外,它们在蛋白质消化和吸收、补体和凝血级联、细胞外基质(ECM)-受体相互作用、细胞因子-细胞因子受体相互作用等信号通路有显著富集。通过构建PPI网络,筛选出16个核心基因:TOP2A、EZH2、NDC80、CCNB1、KIF11、CDC20、TTK、CEP55、BUB1B、HMMR、MELK、RRM2、PBK、NCAPG、KIF15和KIF20A。预后分析表明这16个核心基因的高表达与MPM患者的总生存期差有关。结论TOP2A、EZH2、NDC80、CCNB1、KIF11、CDC20、TTK、CEP55、BUB1B、HMMR、MELK、RRM2、PBK、NCAPG、KIF15和KIF20A等16个核心基因与MPM预后相关,是MPM诊断、治疗的潜在生物标志物。Objective To analyze the potential mechanism and core gene of malignant pleural mesothelioma(MPM)by bioinformatics technology.Methods Gene expression profiles for GSE51024 were first downloaded from the Gene Expression Omnibus(GEO)database.Secondly,differentially expressed genes(DEGs)were screened using the R language package,and DEGs were enriched and analyzed by gene ontology(GO),systematic analysis of gene function and Kyoto encyclopedia of genes and genomes(KEGG).Subsequently,a protein-protein interaction(PPI)network of DEGs was constructed and core genes in the network were selected.Finally,the overall survival of core genes was validated in the Cancer Genome Atlas(TCGA)cohort.Results A total of 574 DEGs were obtained,including 176 up-regulated genes and 398 down-regulated genes.In addition,they were significantly enriched in signaling pathways such as protein digestion and absorption,complement and coagulation cascades,ECM-receptor interactions,cytokine-cytokine receptor interactions.By constructing the PPI network,16 core genes were selected:TOP2A,EZH2,NDC80,CCNB1,KIF11,CDC20,TTK,CEP55,BUB1B,HMMR,MELK,RRM2,PBK,NCAPG,KIF15,and KIF20A.Further prognostic analysis showed that high expression of these 16 core genes were associated with poor overall survival in MPM patients.Conclusion Sixteen core genes,including TOP2A,EZH2,NDC80,CCNB1,KIF11,CDC20,TTK,CEP55,BUB1B,HMMR,MELK,RRM2,PBK,NCAPG,KIF15,and KIF20A,are associated with the prognosis of MPM and are potential biomarkers for the diagnosis and treatment of MPM.

关 键 词：计算生物学 恶性间皮瘤 信号通路 核心基因 

分 类 号：R734.3[医药卫生—肿瘤]