超高效液相色谱-串联三重四极杆质谱法快速测定热带水果饮料中的胭脂虫红  

Rapid determination of carmine cochineal in tropical fruit drinks by ultra performance liquid chromatography-tandem mass spectrometry

作　　者：刘超烽 林志藩 周玉玲 LIU Chao-Feng;LIN Zhi-Fan;ZHOU Yu-Ling(Hainan Institute for Food Control,Haikou 570314,China;Key Laboratory of Tropical Fruits and Vegetables Quality and Safety for State Market Regulation,Haikou 570314,China)

机构地区：[1]海南省食品检验检测中心,海口570314 [2]国家市场监管重点实验室(热带果蔬质量与安全),海口570314

出　　处：《食品安全质量检测学报》2023年第15期113-118,共6页Journal of Food Safety and Quality

基　　金：国家市场监管重点试验室(热带果蔬质量与安全)(ZZ-2022021)。

摘　　要：目的基于超高效液相色谱-串联三重四极杆质谱法(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)建立测定热带水果饮料中胭脂虫红(以胭脂红酸计)的分析方法。方法样品经盐酸超声提取、乙腈稀释、过膜净化,C18色谱柱分离,以乙腈和0.3%甲酸溶液为流动相,梯度洗脱分离,采用串联三重四极杆质谱法对胭脂红酸进行检测和定量分析。结果在5~500 ng/mL质量浓度范围内,线性关系良好,相关系数在0.999以上,方法测定低限为50μg/kg,检出限为10μg/kg;火龙果、百香果、西瓜、莲雾、椰子饮料样品在低(1 mg/kg)、中(5 mg/kg)、高(25 mg/kg)3个浓度加标回收率实验的回收率范围为82.20%~104.11%,相对标准偏差为1.47%~2.43%(n=6)。结论该方法分析速度快、准确度高、重复性好,适用于多种热带水果饮料中胭脂虫红含量的检测。Objective To establish a method for the rapid determination of carmine cochineal(calculated by carminic acid)in tropical fruit drinks by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Methods After extracted by hydrochloric acid with ultrasound,purified by acetonitrile,and separated with membranes,the samples were separated on a C18 column,using acetonitrile and 0.3%formic acid solution as the mobile phase,and detected by triple quadrupole mass spectrometry after gradient elution separation.Results Within the mass concentration range of 5-500 ng/mL,the linear relationship was good and the correlation coefficients were all above 0.999.The limit of quantitation were 50μg/kg,the limit of detection were 10μg/kg,the recoveries of dragon fruit,passion fruit,watermelon,lotus mist,coconut drink samples at low(1 mg/kg),medium(5 mg/kg)and high(25 mg/kg)ranged from 82.20%to 104.11%,and the relative standard deviations were 1.47%to 2.43%(n=6).Conclusion This method is suitable for the rapid determination of carmine cochineal in various tropical fruit drinks during to the advantages including fast analysis speed,high accuracy and good repeatability.

关 键 词：胭脂虫红 超高效液相色谱-串联三重四极杆质谱法 热带水果 饮料 

分 类 号：O657.63[理学—分析化学] TS27[理学—化学]