环丙沙星生物条形码检测技术的研究  

Biological Barcode Detection of Ciprofloxacin

作　　者：栗慧 于苗[1,2,3] 孙丰梅 魏玉文 魏东[1,2,3] LI Hui;YU Miao;SUN Fengmei;WEI Yuwen;WEI Dong(Hebei Key Laboratory of Quality&Safety Analysis-Testing for Agro-Products and Food,Hebei North University,Zhangjiakou 075000,Hebei,China;College of Agriculture and Forestry Science and Technology,Hebei North University,Zhangjiakou 075000,Hebei,China;Zhangjiakou Key Laboratory of Quality&Safety for Characteristics Agro-Products,Hebei North University,Zhangjiakou 075000,Hebei,China;Zhangjiakou Animal Health Supervision Institute,Zhangjiakou 075000,Hebei,China)

机构地区：[1]河北北方学院河北省农产品食品质量安全分析检测重点实验室,河北张家口075000 [2]河北北方学院农林科技学院,河北张家口075000 [3]河北北方学院张家口市特色农产品质量安全重点实验室,河北张家口075000 [4]张家口市动物卫生监督所,河北张家口075000

出　　处：《食品研究与开发》2023年第18期172-178,共7页Food Research and Development

基　　金：河北省重点研发计划项目(19225504D);河北北方学院省属高校基本科研业务费项目(JYT2022010)。

摘　　要：该文对快速检测食品中环丙沙星残留的生物条形码检测技术进行研究。首先将环丙沙星多克隆抗体与条形码DNA链共同修饰在纳米金颗粒上制备纳米金复合探针、环丙沙星单克隆抗体与磁性颗粒结合制备磁性探针,然后对其采用可见光光谱法、透射电镜法进行鉴定;其次将上述探针与环丙沙星标准品进行杂交反应,在磁场的作用下,去除未结合的纳米金复合探针,然后收集标记成功的纳米金复合探针释放出的DNA链,采用微孔板银染的生物条形码技术进行检测,得到环丙沙星残留量。检测环丙沙星的最低检出限为2.13 ng/mL。该方法与常规免疫检测方法相比灵敏度增强,具有实用性,为未来检测小分子物质残留量提供了新思路,也为制备环丙沙星生物条形码检测试剂盒奠定了基础。This study investigates the biobarcode detection technology for rapid detection of enrofloxacin residues in food.Firstly,enrofloxacin polyclonal antibodies and barcode DNA strands are immobilized on gold nanoparticles to prepare nanocomposite probes,while enrofloxacin monoclonal antibodies are conjugated to magnetic particles to prepare magnetic probes.The probes are characterized using visible light spectroscopy and transmission electron microscopy.Then,the probes are hybridized with enrofloxacin standard samples,and unbound nanocomposite probes are removed using a magnetic field.The released DNA strands from the successfully labeled nanocomposite probes are collected,and the residual enrofloxacin is detected using the biological barcode technique with silver staining on a microtiter plate.The method achieves a minimum detection limit of 2.13 ng/mL for enrofloxacin.Compared to conventional immunoassay methods,this approach enhances sensitivity and demonstrates practicality,providing a new strategy for the detection of small molecule residues and laying the foundation for the development of enrofloxacin biobarcode detection kits in the future.

关 键 词：环丙沙星 纳米金复合探针 磁性探针 三明治结构 生物条形码 

分 类 号：TS207.3[轻工技术与工程—食品科学]