种子类中药材油脂过氧化值的荧光半定量检测  

Fluorescence Semi-quantitative Determination of Peroxide Number of Seed Chinese Medicinal Oil

作　　者：叶廷秀 罗红元 范晓萱 米燕 余亚选 陈曦[2] YE Ting-xiu;LUO Hong-yuan;FAN Xiao-xuan;MI Yan;YU Ya-xuan;CHEN Xi(School of Pharmacy,Xiamen Medical College,Xiamen 361023,China;College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China;Fujian Universities and Colleges Engineering Research Center of Marine Biopharmaceutical Resources,Xiamen Medical College,Xiamen 361023,China)

机构地区：[1]厦门医学院药学系,福建厦门361023 [2]厦门大学化学化工学院,福建厦门361005 [3]厦门医学院海洋生物医药资源福建省高校工程研究中心,福建厦门361023

出　　处：《分析测试学报》2023年第9期1188-1193,共6页Journal of Instrumental Analysis

基　　金：厦门市自然科学基金面上资助项目(3502Z20227228)。

摘　　要：该文基于油脂中的过氧化物与9-十八烯基碘化胺(OAmI)发生氧化还原反应,以及OAmI中的碘离子易与铯铅溴钙钛矿纳米晶(CsPbBr3 NCs)中的溴离子发生卤素交换并引起CsPbBr3 NCs荧光发射波长及荧光颜色改变的原理,建立了一种利用荧光发射波长移动检测富含油脂的种子类中药材过氧化值(PON)的方法,并根据钙钛矿纳米晶颜色变化初步制作了荧光半定量比色卡。结果表明,对比国家标准方法,该荧光发射波长移动法检测PON的相对误差在10%左右;通过颜色变化,可快速判断中药材油脂的氧化程度,实现苦杏仁、桃仁等种子类中药材油脂氧化程度的荧光半定量检测。Based on the peroxides in oil undergo an redox reaction with 9-octadecylamine iodide(OAmI),and the iodide ions in OAmI readily undergo halide exchange with bromide ions in cesium lead bromide perovskite nanocrystals(CsPbBr3 NCs)and cause the change of fluorescence emission wavelength and fluorescence color of CsPbBr3 NCs,an analytical method for the determination of peroxide number(PON)of traditional Chinese medicinal materials was established according to the fluorescence emission wavelength shift of CsPbBr3 NCs.Additionally,a semi-quantitative fluorescent color chart has been preliminarily created based on the color change of perovskite nanocrystals.It was found that the relative error for this method when detected the PON was around 10%compared with the national standard method.The oxidation degree of the oil seed Chinese herbal medicine could be quickly judged through the color change.A semi-quantitative analysis for the oxidation degree of seed Chinese herbal medicine such as semen armeniacae amarae and peach kernel was established.

关 键 词：种子类中药材 油脂氧化 过氧化值 荧光半定量法 

分 类 号：O657.3[理学—分析化学] R282[理学—化学]