TGFβ1a regulates zebrafish posterior lateral line formation via Smad5 mediated pathway  

作　　者：Cencan Xing Bo Gong Yu Xue Yanchao Han Yixia Wang Anming Meng Shunji Jia 

机构地区：[1]State Key Laboratory of Biomembrane and Membrane Engineering,Tsinghua-Peking Center for Life Sciences,School of Life Sciences,Tsinghua University,Beijing 100084,China

出　　处：《Journal of Molecular Cell Biology》2015年第1期48-61,共14页分子细胞生物学报（英文版）

基　　金：This work was financially supported by grants from the National Natural Science Foundation of China(#31371460);Major Science Programs of China(#2012CB945101 and#2011CB943800)。

摘　　要：The zebrafish sensory posterior lateral line(pLL)has become an attractive model for studying collective cell migration and cell morphogenesis.Recent studies have indicated that chemokine,Wnt/β-catenin,Fgf,and Delta-Notch signaling pathways participate in regulating pLL development.However,it remains unclear whether TGFβsignaling pathway is involved in pLL development.Here we report a critical role of TGFβ1 in regulating morphogenesis of the pLL primordium(pLLP).The tgfβ1a gene is abundantly expressed in the lateral line primordium.Knockdown or knockout of tgfβ1a leads to a reduction of neuromast number,an increase of inter-neuromast distance,and a reduced number of hair cells.The aberrant morphogenesis in embryos depleted of tgfβ1a correlates with the reduced expression of atoh1a,deltaA,and n-cadherin/cdh2,which are known important regulators of the pLLP morphogenesis.Like tgfβ1a depletion,knockdown of smad5 that expresses in the pLLP,affects pLLP development whereas overexpression of a constitutive active Smad5 isoform rescues the defects in embryos depleted of tgfβ1a,indicating that Smad5 mediates tgfβ1a function in pLLP development.Therefore,TGFβ/Smad5 signaling plays an important role in the zebrafish lateral line formation.

关 键 词：ZEBRAFISH lateral line NEUROMAST TGFβ1a SMAD5 

分 类 号：R73[医药卫生—肿瘤]