制油工艺对三叶木通籽油活性成分及抗氧化活性的影响  

Effects of different processes on active components and antioxidant activities of Akebia trifoliate seed oil

作　　者：高晓龙 徐巍[2] 李颖 周敏[4] GAO Xiaolong;XU Wei;LI Ying;ZHOU Min(Jinan University,Guangzhou,Guangdong 510632,China;Zhongshan Technician College,Zhongshan,Guangdong 528400,China;Guangdong International Joint Research Center for Oil Biorefinery and Nutritional Safety,Guangzhou,Guangdong 510632,China;Institute of Analysis,Guangdong Academy of Sciences,Guangzhou,Guangdong 510070,China)

机构地区：[1]暨南大学,广东广州510632 [2]中山市技师学院,广东中山528400 [3]广东省油料生物炼制与营养安全国际联合研究中心,广东广州510632 [4]广东省科学院测试分析研究所,广东广州510070

出　　处：《食品与机械》2023年第8期155-159,共5页Food and Machinery

摘　　要：目的:比较不同制油工艺对三叶木通籽油活性成分及氧化活性的影响。方法:以三叶木通籽为原料,采用冷榨法、热榨法、超临界CO_(2)萃取法和浸提法4种油脂制取工艺分别制备三叶木通籽油,分析其脂肪酸组成、活性成分(类胡萝卜素、生育酚、多酚、黄酮)含量,以及其清除DPPH自由基的能力。结果:4种工艺制得的三叶木通籽油脂肪酸组成相近,其中棕榈酸含量在20.62%~21.42%,硬脂酸含量在2.68%~3.08%,油酸含量在47.03%~47.37%,亚油酸含量在27.75%~28.07%,其他脂肪酸含量则均未超过0.5%;在活性成分含量方面,冷榨法制取的三叶木通籽油总生育酚、黄酮和多酚含量均最高,分别达到了349.05,103.37,51.78 mg/kg,而浸提法制取的三叶木通籽油总生育酚、黄酮和多酚含量则显著低于其他3种提取方法,仅220.24,57.73,23.45 mg/kg;在DPPH自由基清除能力方面,冷榨法三叶木通籽油的清除效果最佳、浸提法最差。结论:三叶木通籽油富含油酸和亚油酸,且具有清除DPPH自由基能力。Objective:To compare the effects of different oil preparation processes on the active ingredients and oxidation activity of Akebia trifoliate seed oil.Methods:Akebia trifoliata seed oil was prepared from Akebia trifoliata seed by four oil extraction processes:cold pressing,hot pressing,supercritical CO_(2) extraction and extraction.The fatty acid composition,active ingredients(carotenoids,tocopherols,polyphenols,flavonoids)and DPPH free radical scavenging capacity of Akebia trifoliata seed oil were analyzed.Results:The fatty acid composition of Akebia trifoliata seed oil prepared by the four processes was similar.The content of palmitic acid was 20.62%~21.42%,the content of stearic acid was 2.68%~3.08%,the content of oleic acid was 47.03%~47.37%,the content of linoleic acid was 27.75%~28.07%,and the content of other fatty acids did not exceed 0.5%;In terms of the content of active components,the content of total tocopherol,flavonoids and polyphenols in Akebia trifoliata seed oil prepared by cold pressing was the highest,reaching 349.05,103.37 and 51.78 mg/kg respectively,while the content of total tocopherol,flavonoids and polyphenols in Akebia trifoliata seed oil prepared by extraction was significantly lower than that of the other three extraction methods,only 220.24,57.73 and 23.45 mg/kg;In terms of DPPH free radical scavenging capacity,cold pressing Akebia trifoliate seed oil has the best scavenging effect,and the extraction method is the worst.Conclusion:Akebia trifoliate seed oil is rich in oleic acid and linoleic acid,and has the ability of scavenging DPPH free radicals.It can be used as food ingredients,cosmetics and pharmaceutical base oils.

关 键 词：三叶木通 籽油 冷榨法 热榨法 超临界CO_(2)萃取法 浸提法 活性成分 抗氧化活性 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程]