光山油茶饼粕蛋白水解物的制备及功能活性分析  

Preparation and functional activity analysis of protein hydrolysate from Guangshan Camellia oleifera oil extraction residues

作　　者：朱静 吕静 邢淑婕 陈亚蓝 李亚钦 陈龙 ZHU Jing;LU Jing;XING Shujie;CHEN Yalan;LI Yaqin;CHEN Long(College of Food Science,Xinyang Agriculture and Forestry University,Engineering Technology Research Center for Comprehensive Utilization of Characteristic Food Resources in Dabie Mountain,Xinyang 464000)

机构地区：[1]信阳农林学院食品学院,河南省大别山特色食物资源综合利用工程技术研究中心,信阳464000

出　　处：《中国食品添加剂》2023年第9期258-265,共8页China Food Additives

基　　金：河南省重点研发与推广专项(科技攻关)项目(212102110314);河南省青年科学基金项目(212300410228);信阳农林学院高水平科研孵化器建设项目(FCL202014);信阳农林学院科技创新团队(XNKJTD-001);信阳农林学院2019年度学校青年基金项目(2019LG002)。

摘　　要：从光山油茶饼粕中提取蛋白,以油茶粕多肽含量为指标,通过酶法生产油茶饼粕蛋白水解物,并对其功能活性进行验证。采用单因素试验优化酶解时间、加酶量和底物浓度,利用Design Expert 8.0.6统计分析软件得到油茶粕多肽含量随加酶量、底物浓度和酶切时间变化的标准回归模型,采用响应面分析得到最佳酶解条件,并做验证试验。最佳水解条件为:采用胃蛋白酶处理油茶粕,在加酶量6950 U/g、底物浓度2.9%、时间为4.1 h时为最佳酶解条件,油茶粕多肽含量为(6.20±0.15)μg/mL,通过检测发现,油茶粕蛋白多肽对羟自由基的清除能力为93.89%,对DPPH和ABTS+自由基的清除能力分别为61.29%和37.17%,对油脂吸附能力为0.414 g/g,0.20μg/mL的多肽胆酸盐结合能力为0.9μg/mL,说明油茶粕蛋白水解物具有较好的抗氧化和降脂能力。Protein was extracted from Guangshan Camellia oleifera oil extraction residues,and its hydrolysate was prepared by enzymatic method using polypeptide as the index.The functional activity of the protein hydrolysate was tested.The single factor design was used to optimize the enzyme hydrolysis time,the enzyme amount and the concentration of the substrate.The standard regression model was obtained using the Design Expert 8.0.6 statistical analysis software.The optimized enzyme hydrolysis conditions by response surface analysis were as follows:Camellia oleifera meal treated with pepsin,enzyme amount 6950 U/g,substrate concentration 2.9%,and reaction time 4.1 h.The content of polypeptide in camellia oleifera meal was(6.20±0.15)μg/mL.the scavenging ability of protein polypeptide of Camellia oleifera meal to hydroxyl free radical was 93.89%,the scavenging ability to DPPH and ABTS free radical was 61.29% and 37.17%,respectively,and the adsorption ability to oil was 0.414 g/g.The binding capacity of 0.20μg/mL polypeptide cholate was 0.9μg/mL,indicating that the protein hydrolysate of Camellia oleifera meal had good antioxidant and lipid-lowering capacity.

关 键 词：油茶籽粕多肽 响应面分析 抗氧化活性 降脂能力 

分 类 号：TS202.3[轻工技术与工程—食品科学]