Microglial Depletion does not Afect the Laterality of Mechanical Allodynia in Mice  

作　　者：Quan Ma Dongmei Su Jiantao Huo Guangjuan Yin Dong Dong Kaifang Duan Hong Cheng Huiling Xu Jiao Ma Dong Liu Bin Mou Jiyun Peng Longzhen Cheng 

机构地区：[1]School of Life Science and Technology,Harbin Institute of Technology,Harbin 150001,China [2]Shenzhen Key Laboratory of Gene Regulation and Systems Biology,School of Life Sciences,Southern University of Science and Technology,Shenzhen 518055,China [3]Department of Biology,Brain Research Center,Southern University of Science and Technology,Shenzhen 518055,China [4]Institute of Life Science,Nanchang University,Nanchang 330031,China [5]Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions,Shenzhen 518055,China

出　　处：《Neuroscience Bulletin》2023年第8期1229-1245,共17页神经科学通报（英文版）

基　　金：supported by grants from the Ministry of Science and Technology of China(2021ZD0203302);the National Natural Science Foundation of China(32170996,32060199);the Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions(2021SHIBS0002);the Guangdong Science and Technology Committee(2019A1515010041,A2021319);the Shenzhen Innovation Committee of Science and Technology(ZDSYS20200811144002008);the Shenzhen Science and Technology Innovation Committee(JCYJ20180302174233348).

摘　　要：Mechanical allodynia(MA),including punctate and dynamic forms,is a common and debilitating symptom suffered by millions of chronic pain patients.Some peripheral injuries result in the development of bilateral MA,while most injuries usually led to unilateral MA.To date,the control of such laterality remains poorly understood.Here,to study the role of microglia in the control of MA laterality,we used genetic strategies to deplete microglia and tested both dynamic and punctate forms of MA in mice.Surprisingly,the depletion of central microglia did not prevent the induction of bilateral dynamic and punctate MA.Moreover,in dorsal root ganglion-dorsal root-sagittal spinal cord slice preparations we recorded the low-threshold Aβ-fiber stimulation-evoked inputs and outputs of superficial dorsal horn neurons.Consistent with behavioral results,microglial depletion did not prevent the opening of bilateral gates for Aβpathways in the superficial dorsal horn.This study challenges the role of microglia in the control of MA laterality in mice.Future studies are needed to further understand whether the role of microglia in the control of MA laterality is etiology-or species-specific.

关 键 词：MICROGLIA Mechanical allodynia LATERALITY Spinal dorsal horn AΒ Dynamic allodynia Punctate allodynia 

分 类 号：R741[医药卫生—神经病学与精神病学]