植物乳杆菌CCFM 8661调节肠道菌群及缓解便秘研究  

Lactobacillus plantarum CCFM 8661 to regulate intestinal microbiota and relieve constipation

作　　者：张晨玥 马微微[2] 卢慧敏 王琳琳[1] 刘小鸣[1] 王刚[1] 赵建新[1] 张灏[1] ZHANG Chenyue;MA Weiwei;LU Huimin;WANG Linlin;LIU Xiaoming;WANG Gang;ZHAO Jianxin;ZHANG Hao(Jiangnan University,School of Food Science and Technology,Wuxi 214026,China;Heilongjiang University of Chinese Medicine,School of Medicine,Harbin 150006,China)

机构地区：[1]江南大学食品学院,江苏无锡214026 [2]黑龙江中医药大学药学院,黑龙江哈尔滨150006

出　　处：《食品与发酵工业》2023年第18期58-65,共8页Food and Fermentation Industries

基　　金：国家自然科学基金面上项目(32172213)。

摘　　要：益生菌通过调节宿主的肠道菌群来发挥其益生作用,而便秘作为一种常见的胃肠道疾病,其患者会出现肠道菌群紊乱的情况。该文依据《保健食品功能检验与评价方法(2022年版)》中关于调节肠道菌群和润肠通便的检测方法,对植物乳杆菌CCFM 8661调节肠道菌群及缓解便秘功效进行评价。首先应用洛哌丁胺构建便秘小鼠模型,分别以低(1.5×10^(6) CFU/mL)、中(1.5×10^(7) CFU/mL)、高(1.5×10^(8) CFU/mL)3个剂量的植物乳杆菌CCFM 8661灌胃小鼠,检测干预前后小鼠粪便中双歧杆菌、乳杆菌、肠杆菌、肠球菌、产气荚膜梭菌变化情况,并测定便秘相关指标,评价植物乳杆菌CCFM 8661对小鼠肠道菌群的影响及缓解便秘的能力。结果表明,植物乳杆菌CCFM 8661能够调节肠道菌群,且在低剂量条件下,具有缓解便秘的效果。缓解便秘效果可能与其影响便秘相关神经递质,提高便秘小鼠粪便中乙酸含量,以及降低由便秘所引起的炎症有关。Probiotics play a beneficial effect by regulating the intestinal microbiota of the host,and constipation,as a common gastrointestinal disease,causes intestinal microbiota disorders in patients.In this study,Lactobacillus plantarum CCFM 8661 was used to evaluate the effect of regulating intestinal microbiota and relieving constipation based on the methods in“Health Food Function Test and Evaluation Method(2022 Edition)”.First,loperamide was used to construct a constipation mouse model.Firstly,loperamide was applied to construct a constipation mouse model,and mice were gavaged with three doses of L.plantarum CCFM 8661 at low dose(1.5×10^(6) CFU/mL),medium dose(1.5×10^(7) CFU/mL)and high dose(1.5×10^(8) CFU/mL)respectively.Changes of Bifidobacterium,Lactobacillus,Enterobacter,Enterococcus,and Clostridium perfringens in feces of mice before and after intervention were detected,and constipation related indexes were determined to evaluate the effects of L.plantarum CCFM 8661 on intestinal microbiota of mice and its ability to relieve constipation.The results showed that L.plantarum CCFM 8661 could regulate the intestinal microflora of mice,either under normal or disturbed microflora.It also has the effect of relieving constipation at low dose.The constipation-relieving effect might be related to its effect on constipation-related neurotransmitters,the increase of acetic acid content in stool of constipated mice,and the decrease of inflammation caused by constipation.

关 键 词：植物乳杆菌 肠道菌群 便秘 胃肠调节递质 炎症因子 

分 类 号：TS201.3[轻工技术与工程—食品科学]