铈基介孔金属有机骨架固定化酶的构建及催化性能  

Construction and Catalytic Performance of Cerium-Based Mesoporous MOF Immobilized Enzymes

作　　者：周晓彤 许俊洋 姜艳军[1] 马丽[1] 周丽亚[1] 郑晓冰[1] 贺莹[1] Zhou Xiaotong;Xu Junyang;Jiang Yanjun;Ma Li;Zhou Liya;Zheng Xiaobing;He Ying(School of Chemical Engineering and Technology,Hebei University of Technology,Tianjin,300130)

机构地区：[1]河北工业大学化工学院,天津300130

出　　处：《化学通报》2023年第9期1103-1111,1025,共10页Chemistry

基　　金：国家自然科学基金项目(22178083和21878068);河北省重点研发计划项目(21372804D和20372802D)资助。

摘　　要：金属有机骨架(MOF)材料由于其孔隙率高、比表面积大以及具有发达的内联通孔道结构等优点,可以作为优良的生物分子固定化载体。通过表面活性自组装策略制备了铈基介孔MOF(Ce-MOF-F),表征结果表明,该材料有大的比表面积和呈辐射状的介孔孔道结构。以其为载体、南极假丝酵母脂肪酶B(CALB)为模型酶,通过物理吸附法制备了生物催化剂CALB@Ce-MOF-F,对该固定化酶的酶载量和催化性能进行了研究。在优化条件下,CALB的负载量为162.0mg/g载体,水解活性为899.1U/g蛋白。与游离CALB相比,CALB@Ce-MOF-F表现出对高温、酸碱和有机溶剂等有更强的耐受性;将Ce-MOF-F用于多种酶的固定化,研究其作为载体的普适性,结果表明,介孔Ce-MOF-F对洋葱伯克氏菌脂肪酶(BCL)和漆酶有良好的固定效果,可以作为良好载体,并能对酶起到较好的保护作用。Metal-organic framework(MOF)materials have been used as excellent supports for immobilized enzymes due to their high porosity,large specific surface area and well-developed inline pore structure.The Ceria-based mesoporous MOF(Ce-MOF-F)was prepared by surface-active self-assembly strategy.Its large specific surface area and pore structure were favorable for enzyme adsorption in Ce-MOF-F.The biocatalyst CALB@Ce-MOF-F was prepared by adsorption using Ce-MOF-F as the support and Candida antarctica lipase B(CALB)as the model enzyme.The enzyme loading and catalytic performance of the immobilized enzyme were investigated.Under the optimized conditions,the loading amount of CALB was 162.0 mg/g.upon,and the hydrolytic activity was 899.1 U/gprotin.CALB@Ce-MOF-F had exhibited greater tolerance to high temperature,acids,and alkalis as well as organic solutions compared to free CALB.Furthermore,Ce-MOF-F was used for the immobilization of various enzymes to study its general applicability as a carrier,and the results showed that Ce-MOF-F had good immobilization effect on immobilized Burkholderia cepacia lipase(BCL)and laccase,which proved that it could be a suitable carrier for the immobilization of enzymes and provide good protection.

关 键 词：金属有机骨架 铈基介孔材料 固定化酶 脂肪酶 

分 类 号：TQ426.97[化学工程]