基于超滤-HPLC-MS/MS技术的苦荞提取物抑制线粒体肿胀活性研究  

The Inhibitory Activity of Tartary Buckwheat Extracts Against Mitochondrial Swelling Through Ultrafiltration-HPLC-MS/MS

作　　者：江林娟 韩林 李云龙 王敏[2] Jiang Linjuan;Han Lin;Li Yunlong;Wang Min(College of Life Sciences,Northwest A&F University,Yangling 712100;College of Food Science and Engineering,Northwest A&F University,Yangling 712100;Institute of Functional Food of Shanxi,Shanxi Agricultural University,Taiyuan 030031)

机构地区：[1]西北农林科技大学生命科学学院,杨凌712100 [2]西北农林科技大学食品科学与工程学院,杨凌712100 [3]山西农业大学山西功能食品研究院,太原030031

出　　处：《中国粮油学报》2023年第8期117-123,共7页Journal of the Chinese Cereals and Oils Association

基　　金：陕西省重点研发计划项目(2022NY-002);国家现代农业产业技术体系项目(CARS-07-E2-01)。

摘　　要：为了阐明苦荞对肝脏线粒体肿胀的抑制作用和物质基础,采用超滤技术,结合HPLC-MS/MS分析,研究苦荞中靶向肝脏线粒体发挥活性的功能成分。结果表明,苦荞含有丰富的总酚和总黄酮,其中以乙醇-麸皮粉提取物(YF)中总酚和总黄酮含量最高,为(32.48±0.37)mg GA/g DW和(63.06±0.21)mg rutin/g DW,丙酮和水提取物次之。同时,YF对FeSO 4-VC诱导的肝脏线粒体肿胀抑制效果最好,超滤-HPLC-MS/MS分析发现,苦荞YF中发挥肝脏线粒体肿胀抑制作用的主要活性成分为槲皮素、山奈酚、大黄素和L-苹果酸,其中槲皮素和山奈酚的效果优于大黄素和L-苹果酸。细胞实验进一步证实,槲皮素和山奈酚可有效恢复高脂诱导的线粒体膜电位下降,降低ROS水平,发挥线粒体保护作用。In order to explore the inhibitory activity and compound basis of Tartary buckwheat against hepatic mitochondrial swelling,ultrafiltration-HPLC-MS/MS was employed to investigate the functional substances for protecting hepatic mitochondria.The results indicated that the extracts of Tartary buckwheat in different parts contained plenty of total polyphenols and total flavonoids.Wherein,ethanol-bran extract(YF)had the highest content,reaching(32.48±0.37)mg GA/g DW and(63.06±0.21)mg rutin/g DW,respectively,followed by acetone and water extract.Meanwhile,YF showed the best effect to inhibit FeSO 4-VC induced hepatic mitochondrial swelling,and quercetin,kaempferol,emodin and L-malic acid were identified as the main active compounds that response for the inhibitory activity of mitochondrial swelling by ultrafiltration-HPLC-MS/MS analysis.Moreover,quercetin and kaempferol revealed much better inhibitory effect than that of emodin,and L-malic acid.Cellular experiments further verified that these compounds,especial quercetin and kaempferol,could effectively restore high fat-induced mitochondrial membrane potential and significantly decreased the ROS level in HepG2 cells.

关 键 词：苦荞 超滤技术 高效液相色谱-质谱联用(HPLC-MS/MS) 线粒体肿胀 

分 类 号：TS201[轻工技术与工程—食品科学]