羊肚菌蛋白抗氧化肽的制备及抗运动疲劳活性研究  

Preparation and antifatigue activity of antioxidant peptide from Morchella esculenta protein

作　　者：马建伟[1] MA Jianwei(College of Physical Education,Henan University of Science and Technology,Luoyang 471000,China)

机构地区：[1]河南科技大学体育学院,河南洛阳471000

出　　处：《中国酿造》2023年第9期163-169,共7页China Brewing

基　　金：2018年河南省教育厅项目(2018-ZZJH-170)。

摘　　要：该研究以羊肚菌蛋白为原料,采用酶解法制备羊肚菌蛋白抗氧化肽,通过单因素试验响应面法优化其酶解工艺条件,并考察其抗氧化活性及抗运动疲劳活性。结果表明,最佳的水解酶为食用菌水解酶A1,最佳酶解工艺条件为食用菌水解酶A1添加量0.15%、底物质量分数4.80%、酶解温度49℃、酶解时间116 min、酶解pH值4.4。在此优化条件下,羊肚菌蛋白抗氧化肽对DPPH·、·OH和·O_(2)-的清除率分别为91.34%、85.04%、63.90%。抗运动疲劳活性试验结果表明,与空白对照组相比,高剂量组小鼠的力竭游泳时间、爬杆时间和耐缺氧时间延长最长,分别延长15.67 min、53.99 min和28.51 min;且其体内的血糖和糖原水平升高,血清尿素氮含量降低2.3 mmol/L、血乳酸含量降低2.93 mmol/L,表明羊肚菌蛋白抗氧化肽具有一定的抗运动疲劳作用。相关性试验表明,羊肚菌蛋白抗氧化多肽的抗氧化与抗疲劳作用具有一定相关性。Using Morchella esculenta protein as raw material,the M.esculenta protein antioxidant peptides were prepared by enzymatic hydrolysis method,the enzymatic hydrolysis process conditions were optimized using single factor tests and response surface methodology,and the antioxidant activity and anti-fatigue activity was investigated.The results showed that the optimal hydrolase was hydrolase A1 from edible fungi.The optimum enzymatic hydrolysis conditions were as follows:hydrolase A1 addition 0.15%,substrate mass fraction 4.80%,enzymatic hydrolysis temperature 49℃,time 116 min,and pH 4.4.Under these optimized conditions,the scavenging rates of M.esculenta antioxidant peptides on DPPH·,·OH and·O_(2)-were 91.34%,85.04%and 63.90%,respectively.The results of antifatigue activity tests showed that compared with the blank control group,the mice in high-dose group had the longest prolongation of exhausted swimming time,pole climbing time,and hypoxia tolerance time,which were 15.67 min,53.99 min,and 28.51 min,respectively.Moreover,the level of blood sugar and glycogen in the body increased,the content of serum urea nitrogen decreased 2.3 mmol/L,and the content of blood lactic acid decreased 2.93 mmol/L,indicating that M.esculenta protein antioxidant peptides had a certain anti-fatigue effect.The correlation test showed that the antioxidation and antifatigue effects of the M.esculenta protein antioxidant peptides were related to a certain extent.

关 键 词：羊肚菌蛋白 食用菌水解酶 响应面法 抗氧化肽 抗运动疲劳 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]