液质联用法检测动物源食品中氟苯尼考和氟苯尼考胺残留量  

Determination of florfenicol and florfenicol amine residues in animal-derived food by liquid chromatography-mass spectrometry

作　　者：汪琼[1] 严付华[1] 白祖海[1] 胡骢 WANG Qiong;YAN Fuhua;BAI Zuhai;HU Cong(Changsha Municipal Center for Disease Control and Prevention,Changsha,Hunan 410004,China)

机构地区：[1]长沙市疾病预防控制中心,湖南长沙410004

出　　处：《实用预防医学》2023年第9期1138-1140,共3页Practical Preventive Medicine

摘　　要：目的利用超高效液相色谱—串联质谱联用仪建立一种高效、简便、快捷检测动物源食品中氟苯尼考和氟苯尼考胺残留方法。方法经过优化样品前处理程序,动物源食品样品经氨化乙腈提取,采用C18固相萃取填料净化,超高效液相色谱—串联质谱测定,内标法定量。结果本方法中氟苯尼考和氟苯尼考胺检出限均为:0.5μg/kg,定量限均为:1.5μg/kg。在1.0~40.0μg/L范围内呈良好线性关系,且3个添加浓度回收率均能达到75%以上,相对偏差小于10%。结论该方法采用氨化乙腈提取目标物,提高了样品前处理回收率,方法高效简便、准确可靠,可用于大批量动物源食品中氟苯尼考和氟苯尼考胺残留量检测。Objective To establish an efficient,simple and rapid method for the detection of florfenicol and florfenicol amine residues in animal-derived food by ultra high performance liquid chromatography-tandem mass spectrometry.Methods The optimization of sample pretreatment procedures as follows:the food samples were extracted by ammoniation acetonitrile and purified by C18 solid phase extraction packing.After that,the extraction was determined by ultra high performance liquid chromatography-tandem mass spectrometry and quantified by internal standard method.Results In this method,the limits of detection of florfenicol and florfenicol amine were both 0.5μg/kg,and the limits of quantitation were both 1.5μg/kg.A good linear relationship was obtained with the concentration ranged from 1.0μg/L to 40.0μg/L,and the recoveries of three different spiked concentrations were all above 75%,with the relative deviation being less than 10%.Conclusion Ammoniation acetonitrile was selected to extract the food samples in this method,which increased the sample pretreatment recoveries.This method is high-efficient,simple,accurate and reliable,which can be used for detecting florfenicol and florfenicol amine residues in animal-derived food.

关 键 词：氟苯尼考 氟苯尼考胺 动物源食品 超高效液相色谱—串联质谱 

分 类 号：R155.6[医药卫生—营养与食品卫生学]