田葛缕子叶绿体基因组微卫星特征分析  

Characterization Analysis of Microsatellite in Chloroplast Genome of Carum buriaticum Turcz

作　　者：李昂 金亮[2] 朱惠琴[1] 沈宁东[1] LI Ang;JIN Liang;ZHU Huiqin;SHEN Ningdong(College of Agriculture and Animal Husbandry,Qinghai University,Xining,Qinghai 810016;Institute of Landscape Plants and Flower,Zhejiang Academy of Agricultural Sciences,Hangzhou,Zhejiang 311251)

机构地区：[1]青海大学农牧学院,青海西宁810016 [2]浙江省农业科学院浙江省园林植物与花卉研究所,浙江杭州311251

出　　处：《北方园艺》2023年第16期96-100,共5页Northern Horticulture

基　　金：国家自然科学基金地区科学基金资助项目(32160092);青海省科技厅基础资助项目(2018-ZJ-752)。

摘　　要：以田葛缕子幼嫩叶片为试材,采用CTAB法提取叶绿体DNA,测定并分析田葛缕子叶绿体全基因组序列(153697 bp),旨在研究田葛缕子叶绿体基因组微卫星(cpSSR)特征,为田葛缕子资源分子鉴定及遗传多样性研究提供参考依据。结果表明:MISA软件在田葛缕子叶绿体基因组序列中共识别出183个SSR位点,位点间平均分布距离为839.90 bp。其中109个cpSSR(占SSR位点总数的59.60%)主要集中于大单拷贝区(LSC),57.69%的SSR位点的重复序列主要以单碱基重复序列为主,且主要由A、T碱基组成。cpSSR长度主要为8~15 bp(占78.69%),大于15 bp的cpSSR共有31个,田葛缕子叶绿体基因组上的cpSSR位点具有多态性潜能。The chloroplast DNA was extracted by CTAB method from the young leaves of Carum buriaticum Turcz.,and the whole chloroplast genome sequence(153697 bp)of C.buriaticum Turcz.was determined and analyzed,in order to study the characteristics of chloroplast microsatellite(cpSSR)of C.buriaticum Turcz.chloroplast genome,and to provide theoretical reference for the molecular identification and genetic diversity of C.buriaticum Turcz.resources.The results showed that a total of 183 SSR loci were identified by MISA software in the chloroplast genome sequence of C.buriaticum Turcz.and the average distribution distance between loci was 839.90 bp.cpSSR was mainly distributed in the large singlE-copy region(LSC),and the number of cpSSR in this region was 109,accounting for 59.60%of the total.The most common repeat type of the cpSSR was mononucleotide repeat,accounting for 57.69%.The length of cpSSR distributed from 8-15 bp and accounted for 78.69%.There were 31 cpSSR of length greater than 15 bp,indicating that the cpSSR of C.buriaticum Turcz.had the potency of high polymorphism.The cpSSR loci in chloroplast genome of C.buriaticum Turcz.had potential of polymorphism.

关 键 词：田葛缕子 叶绿体基因组 微卫星 CPSSR 

分 类 号：S567.239[农业科学—中草药栽培]