基于金@铂纳米酶的食品中过氧化氢残留快速检测试纸的研制  

Preparation of Test Strip Based on Au@Pt Nanozymes for Rapid Detection of Hydrogen Peroxide Residues in Foods

作　　者：李博然 成璐瑶 曹智鸿 程楠 罗云波 LI Boran;CHENG Luyao;CAO Zhihong;CHENG Nan;LUO Yunbo(Beijing Laboratory for Food Quality and Safety,College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China;Key Laboratory of Safety Assessment of Genetically Modified Organism(Food Safety),Ministry of Agriculture and Rural Affairsy,Beijing 100083,China)

机构地区：[1]中国农业大学食品科学与营养工程学院,食品质量与安全北京实验室,北京100083 [2]农业农村部农业转基因生物安全评价(食用)重点实验室,北京100083

出　　处：《食品科学》2023年第18期381-388,共8页Food Science

基　　金：中国农业大学2115人才工程项目;山东省重点研发计划项目(2019JZZY011014)。

摘　　要：为实现对食品中残留过氧化氢便捷、灵敏的检测,研制一种基于金@铂纳米酶的过氧化氢快速检测试纸。该试纸以Whatman No.1滤纸为载体,以金@铂纳米酶为催化剂,以3,3’,5,5’-四甲基联苯胺为显色剂,以pH 4.6的醋酸-醋酸钠溶液为缓冲体系制备而成。通过优化试纸制作工艺,确定试纸浸泡时间为10 s,最快显色稳定时间为3 min。该试纸检出限为0.34 mg/kg,具有较好的线性关系(R^(2)=0.9931)。该试纸制作方便、准确度高、稳定性好、操作简单,可以应用于食品各流通环节的即时现场检测。A gold@platinum(Au@Pt)nanozyme-based test strip for the convenient and sensitive detection of hydrogen peroxide residues in foods was developed using Whatman No.1 filter paper as the carrier,Au@Pt nanozyme as the catalyst,3,3’,5,5’-tetramethylbenzidine as the color developer,and acetic acid-sodium acetate solution at pH 4.6 as the buffer system.The optimized test strip soaking time was 10 s.The test paper could detect hydrogen peroxide in samples within 3 min with a minimum detection limit of 0.34 mg/kg and a good linearity(R^(2)=0.9931).The test paper is easy to make,highly accurate,stable and simple to operate,and can be used in the real-time on-site detection of food products in various distribution channels.

关 键 词：过氧化氢 快速检测 试纸法 纳米酶 泡椒制品 

分 类 号：TS207.3[轻工技术与工程—食品科学]