生鲜食品致腐假单胞菌生物被膜形成及其调控机制研究进展  

Biofilm Formation and Its Regulatory Mechanism in Pseudomonas,a Spoilage Bacterium in Fresh Foods

作　　者：鲁重 刘静聪 吴诗媛 冯立芳 陈剑 朱军莉 LU Zhong;LIU Jingcong;WU Shiyuan;FENG Lifang;CHEN Jian;ZHU Junli(Food Safety Key Laboratory of Zhejiang Province,School of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou 310018,China)

机构地区：[1]浙江工商大学食品与生物工程学院,浙江省食品安全重点实验室,浙江杭州310018

出　　处：《食品科学》2023年第17期278-285,共8页Food Science

基　　金：“十三五”国家重点研发计划专项课题(2019YFD0901602);省属高校基本科研业务费项目(XRK21001)。

摘　　要：假单胞菌是低温冷藏动物源性食品和果蔬的重要腐败菌,会造成食品品质劣变,从而导致严重的经济损失。致腐假单胞菌的致腐性与生物被膜形成密切相关,生物被膜能够增强该菌在食品加工和贮藏环境的生存和适应能力,引起持续和交叉污染。然而,与致腐相关的假单胞菌生物被膜形成及其机制仍缺乏系统的总结。本文介绍了生鲜食品中致腐假单胞菌的污染情况,分析了假单胞菌生物被膜形成及胞外基质的组成,重点阐述了致腐假单胞菌生物被膜形成的环鸟苷二磷酸、群体感应和小RNA分子等主要调控机制,为揭示生鲜食品来源的假单胞菌致腐机制及有效控制其生物被膜的污染提供理论参考。Pseudomonas,an important spoilage bacterium in animal-derived foods as well as fruits and vegetables in the cold chain,can cause huge economic losses to the food industry.The food spoilage ability of Pseudomonas is closely related to the formation of biofilm,which can enhance the survival and adaptability to the food processing and storage environment of Pseudomonas,causing persistent contamination.However,a systematic review of the biofilm formation of Pseudomonas and its mechanism is still lacking at present.This article describes the contamination status of Pseudomonas in fresh foods,analyzes the biofilm formation and extracellular matrix composition of Pseudomonas,and elucidates the major regulatory factors of the biofilm formation of Pseudomonas such as cyclic diguanylate,quorum sensing system and small RNAs.We believe that this review will provide a theoretical reference for uncovering the mechanism of food spoilage caused by Pseudomona and controlling its biofilm contamination.

关 键 词：假单胞菌 生物被膜 致腐 调控机制 信号通路 

分 类 号：TS201.3[轻工技术与工程—食品科学]