植物乳杆菌与纤维素酶组合对玉米秸秆微贮品质的影响  

Effect of Lactobacillus plantarum and Cellulase Combination on The Fermented Quality of Corn Straw

作　　者：王加黛 王利军 王平[1] 刘超齐 金三俊 党晓伟 常娟[1] 尹清强[1] WANG Jiadai;WANG Lijun;WANG Ping;LIU Chaoqi;JIN Sanjun;DANG Xiaowei;CHANG Juan;YIN Qingqiang(College of Animal Science and Technology,Henan Agricultural University,Henan Zhengzhou 450046,China;Henan Delin Biological Products Co.,Ltd.,Henan Xinxiang 453500,China)

机构地区：[1]河南农业大学动物科技学院,河南郑州450046 [2]河南德邻生物制品有限公司,河南新乡453500

出　　处：《饲料工业》2023年第19期90-94,共5页Feed Industry

基　　金：河南省创新示范专项[201111311100];河南省研究生教育改革与质量提升工程项目[YJS2023JD18];新乡市重大科技专项[22ZD011]。

摘　　要：为了提高微贮玉米秸秆的发酵质量,以玉米秸秆为主要发酵底物,利用三因素三水平正交试验筛选出发酵的最佳条件;在此基础上采用单因素设计,分为对照组(不加菌酶)、单菌组(植物乳杆菌1×10^(6)CFU/g)、单酶组(纤维素酶0.1 FPU/g风干物质)、菌酶复合组(植物乳杆菌1×10^(6)CFU/g、纤维素酶0.1 FPU/g风干物质)进行玉米秸秆微贮。结果表明:发酵底物风干物质的配比为玉米秸秆96%、玉米粉2%、棉粕2%、水分含量为60%时微贮效果最好。在此条件下菌酶复合室温微贮28 d后微贮秸秆品质评分最高,玉米秸秆pH降至3.88,显著低于对照组(P<0.05),乳酸菌活菌数显著高于对照组(P<0.05),中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)、纤维素及半纤维素含量显著低于对照组(P<0.05)。说明菌酶组合能够提高玉米秸秆的微贮品质。The aim of this study was to increase microbial fermented quality of corn straw,the optimal fermented conditions were selected by orthogonal design with three factors and three levels based on the main substrate of corn straw.After the optimal fermented condition was obtained,the further fermenting experiment was divided into three groups:the control group(without adding Lactobacillus plantarum and cellulase),single bacterial group(Lactobacillus plantarum 1×10^(6) CFU/g),single enzyme group(cellulase 0.1 FPU/g air-dried matter),enzyme-bacteria group(Lactobacillus plantarum 1×10^(6) CFU/g+cellulase 0.1 FPU/g air-dried matter).The results showed that the optimal composition of fermented substrates in dry matter was 96%corn straw,2%corn meal and 2%cottonseed meal,plus 60%moisture,in which the best fermenting result was obtained.Based on the above fermented substrates,the highest score of fermentation was obtained after 28 days fermentation including both bacteria and enzyme additions,in which pH value(3.88)was significantly decreased(P<0.05),the counts of lactic acid bacteria were significantly increased(P<0.05),the contents of NDF,ADF,cellulose and hemicellulose were significantly decreased(P<0.05),compared with the control group.In conclusion,the enzyme-bacteria combination can improve the fermentation quality of corn straw.

关 键 词：玉米秸秆 微贮 发酵 植物乳杆菌 纤维素酶 

分 类 号：S816.9[农业科学—饲料科学]