氟磺胺草醚胁迫红小豆幼苗代谢物及通路分析  

Analysis of Metabolites and Pathways in Adzuki Bean Seedlings under Fomesafen Stress

作　　者：杨建[1,2] 汤华成 曹冬梅[1,2,3,4] 崔航 娄雨豪[1,2] 王冀菲 张东杰 Yang Jian;Tang Huacheng;Cao Dongmei;Cui Hang;Lou Yuhao;Wang Jifei;Zhang Dongjie(College of Food Science,Heilongjiang Bayi Agricultural University,Daqing 163319,Heilongjiang,China;Key Laboratory of Agro-Products Processing and Quality Safety of Heilongjiang Province,Daqing 163319,Heilongjiang,China;Heilongjiang Engineering Research Center for Coarse Cereals Processing and Quality Safety,Daqing 163319,Heilongjiang,China;National Coarse Grain Engineering Research Center,Daqing 163319,Heilongjiang,China;Beidahuang Modern Agricultural Industrial Technology Provincial Cultivation Collaborative Innovation Center,Daqing 163319,Heilongjiang,China)

机构地区：[1]黑龙江八一农垦大学食品学院,黑龙江大庆163319 [2]黑龙江省农产品加工与质量安全重点实验室,黑龙江大庆163319 [3]黑龙江省杂粮加工及质量安全工程技术研究中心,黑龙江大庆163319 [4]国家杂粮工程技术研究中心,黑龙江大庆163319 [5]北大荒现代农业产业技术省级培育协同创新中心,黑龙江大庆163319

出　　处：《作物杂志》2023年第4期230-236,共7页Crops

基　　金：国家重点研发计划(2018YFE0206300);杂粮及制品安全风险评估及标准体系建设项目(2018YFE0206300-10);黑龙江省优势特色学科资助项目(黑教联[2018]4号)。

摘　　要：为探究田间喷施氟磺胺草醚(FSA)对红小豆幼苗生长代谢的调控机制,以是否喷施FSA的红小豆幼苗为试验材料,采用液相色谱-质谱联用(LC-MS)代谢组学技术对红小豆幼苗代谢物的变化进行分析。结果表明,喷药的红小豆幼苗(Z-2-ZZ-2)组与未喷药红小豆幼苗(Z-2-ZZ)组相比,正离子模式下筛选出显著变化的差异代谢物106种(上调50种,下调56种),负离子模式下130种(上调42种,下调88种),正离子模式下注释到差异显著的代谢通路5条(嘧啶代谢、异黄酮生物合成、嘌呤代谢、半乳糖代谢、精氨酸和脯氨酸代谢),映射到差异代谢物13种,负离子模式下注释到差异显著的代谢通路2条(花青素生物合成和黄酮类生物合成),映射到差异代谢物5种。苯丙烷和聚酮化合物、脂质、类脂分子、有机酸及其衍生物类化合物在红小豆幼苗抵御FSA的胁迫中起主要作用。本研究为红小豆田间使用FSA的安全性评价及红小豆增收增产提供新思路。To explore the regulatory mechanism of field spraying fomesafen(FSA)on the growth and metabolism of adzuki bean seedlings,using the adzuki bean seedlings sprayed with or without FSA as the experimental material,the metabolomics of adzuki bean seedlings was analyzed by liquid chromatography-mass spectrometry(LC-MS).The results showed that compared with the unsprayed adzuki bean seedlings(Z-2-ZZ-2)group,the significantly changed differential metabolites were screened,106 in the cation mode(50 up-regulated,56 down-regulated),130 in anion mode(42 up-regulated,88 down-regulated),five metabolic pathways with significant differences(pyrimidine metabolism,isoflavone biosynthesis,purine metabolism,galactose metabolism,and arginine and proline metabolism)were annotated in cation mode,mapped to 13 differential metabolites,and annotated to two significantly different metabolic pathways(anthocyanin biosynthesis and flavonoid biosynthesis)in anion mode,mapped to five differential metabolites.Phenylpropane and polyketides,lipids and lipid molecules,and organic acids and their derivatives played a major role in the resistance of adzuki bean seedlings to FSA stress.It provided new ideas for the safety evaluation of the use of FSA in the field of adzuki bean and for increasing the income and yield of adzuki bean.

关 键 词：红小豆 幼苗 氟磺胺草醚(FSA) 液相色谱―质谱联用技术(LC-MS) 代谢物 代谢通路 

分 类 号：S521[农业科学—作物学]