Qizhi Jiangtang capsule(芪蛭降糖胶囊)activates podocyte autophagy in diabetic kidney disease by inhibiting phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin pathways  

作　　者：GUO Zhaoan SUN Lina LIU Yingying LI Ruifeng LIU Chong DIAO Ke SHI Jing SUN Jun 

机构地区：[1]Department of Nephrology,Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250011,China [2]Department of Nephrology,Linyi People’s Hospital,Linyi 276003,China [3]Shandong University of Traditional Chinese Medicine,Jinan 250355,China [4]Shandong GuoxinYiyang Group Zibo Hospital,Zibo 255051,China

出　　处：《Journal of Traditional Chinese Medicine》2023年第4期667-675,共9页中医杂志（英文版）

基　　金：National Natural Science Foundation of China Project:Experimental Research on Podocyte Autophagy of Diabetic Nephropathy Regulated by Qizhi Jiangtang Capusul(No.81874440);Natural Science Foundation of Shandong Province Project:Curcumin Ameliorates Diabetic Nephropathy via Regulating the Intestinal Barrier-Inflammation“cross-talk”(ZR2020QH063)。

摘　　要：OBJECTIVE:To investigate the therapeutic action and mechanism of the Qizhi Jiangtang capsule(芪蛭降糖胶囊,QZJT)on diabetic kidney disease(DKD)treatment.METHODS:This experiment used db/db mice and podocytes(MPC5)to develop DKD model.Evaluation of the effect of the QZJT on db/db mice by testing urine and blood biochemical parameters(24-h urinary albumin,serum creatinine,blood urine nitrogen),pathological kidney injury,and podocyte integrity.Moreover,autophagosomes in podocytes of DKD mice and cultured podocytes were detected using electron microscopy.Additionally,Western blotting was applied to detect the expression of podocyte marker protein(podocin),autophagy-associated proteins,and phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin(PI3K/AKT/mTOR)signaling pathway changes in vivo and in vitro.RESULTS:QZJT significantly reduced urine protein,blood nitrogen urea,and serum creatinine and showed histological restoration of renal tissues.QZJT also significantly improved the down-regulation of podocin and foot fusion and effacement in db/db mice.QZJT increased autophagic vesicles in mice and cultured podocytes.QZJT also upregulated microtubuleassociated protein 1 light chain 3-II(LC3-II)/(LC3-I)and Beclin-1 and downregulated phosphorylated-PI3K(pPI3K),p-AKT,and p-mTOR in db/db mice and MPC5 cells.However,autophagy inhibitor 3-methyladenine partially alleviated the above effects in MPC5 cells.CONCLUSIONS:These results showed that the QZJT can enhance podocyte autophagy and ameliorate podocyte injury in DKD by inhibiting the PI3K/AKT/mTOR signaling pathway.

关 键 词：diabetic kidney disease PODOCYTES AUTOPHAGY AKT Qizhi Jiangtang capsule 

分 类 号：R285.5[医药卫生—中药学]