山药皮多糖的分离纯化、结构特征及体外抗氧化活性研究  

Study on Separation,Purification,Structural Characteristics and in Vitro Antioxidant Activity of Polysaccharides from Chinese Yam Peel

作　　者：叶美芝 伍紫仪 吴金松 钟青萍[1] YE Meizhi;WU Ziyi;WU Jinsong;ZHONG Qingping(Guangdong Provincial Key Laboratory of Food Quality and Safety,College of Food Science,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]广东省食品质量安全重点实验室,华南农业大学食品学院,广东广州510642

出　　处：《食品工业科技》2023年第19期78-85,共8页Science and Technology of Food Industry

基　　金：国家自然科学基金面上项目(31972046);广东省自然科学基金项目(2021A1515011083);广东省科技计划项目(2020B1212060059)。

摘　　要：本研究通过三氯乙酸法、Sevag法以及Sevag-木瓜蛋白酶法,研究了山药皮粗多糖的除蛋白工艺,对提纯后的山药皮多糖组分进行红外光谱和单糖组分等结构特征分析,并对体外抗氧化活性进行测定。研究结果表明:提取山药皮多糖的适宜的乙醇终浓度为90%,多糖得率为9.55%,酶结合Sevag法为最优的脱蛋白工艺,在此条件下多糖保留率为64.7%,蛋白脱除率为76.8%。山药皮多糖经过DEAE-52纤维素柱层析后得到CYPP-1和CYPP-2两种主要多糖组分,其分子量分别为4.442 kDa和4.278 kDa。CYPP-1由盐酸氨基葡萄糖、半乳糖、葡萄糖、甘露糖和葡萄糖醛酸组成,摩尔比为:11.1:14.4:21.8:35:10.8;CYPP-2由盐酸氨基葡萄糖、甘露糖和葡萄糖醛酸组成,摩尔比为:10.5:47.9:16.8。红外图谱分析显示两种组分均符合植物多糖结构特征,均为α-型多糖。综合比较不同浓度的CYPP-1和CYPP-2对DPPH、羟自由基、ABTS+和超氧阴离子自由基的体外清除效果,其中8.0 mg/mL的CYPP-1的清除率分别为89.83%、46.72%、67.27%、51.20%,其抗氧化活性高于CYPP-2。In this study,the optimal process for the deproteinization of crude polysaccharides from Chinese yam peel was investigated by using the trichloroacetic acid method,Sevag method and Sevag-papain enzyme method.The purified polysaccharide fractions were characterized by infrared spectroscopy,the monosaccharide composition analysis,and the antioxidant activity in vitro was determined.The results indicated that the optimal ethanol final concentration was 90%,the yield of polysaccharide reached 9.55%,and polysaccharide precipitation rate was the highest.The better deproteinization process was papain treatment combined with Sevag method.Under the condition,the polysaccharide retention rate reached 64.7%and the deproteinization rate was 76.8%.Two major polysaccharide fractions named CYPP-1 and CYPP-2 were isolated and purified from crude polysaccharide by DEAE-52 cellulose column chromatography,and their molecular weights were 4.442 kDa and 4.278 kDa respectively.CYPP-1 was consisted of glucosamine hydrochloride,galactose,glucose,mannose and glucuronic acid with the molar ratio of 11.1:14.4:21.8:35:10.8,and CYPP-2 was consisted of glucosamine hydrochloride,mannose and glucuronic acid with the molar ratio of 10.5:47.9:16.8.Infrared spectroscopy showed that both fractions were consistent with the structural characteristics of plant polysaccharides,beingα-type polysaccharides.The scavenging effects of different concentrations of CYPP-1 and CYPP-2 on DPPH,hydroxyl radicals,ABTS+and superoxide anion radicals in vitro were compared,and the scavenging rates of 8.0 mg/mL CYPP-1 were 89.83%,46.72%,67.27%and 51.20%,respectively.And its antioxidant activity was higher than that of CYPP-2.

关 键 词：山药皮 多糖 分离纯化 结构特征 抗氧化活性 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]