接骨木镰刀菌产呕吐毒素条件筛选及致病性分析  

Screening of Conditions for Vomitoxin Production by Fusarium sambucinum and Pathogenicity Analysis

作　　者：肖萌萌 贺付蒙 冯旭[1] 徐永清[1] 蒋先锋[1] 王雪 刘丹[1] 李凤兰[1] XIAO Mengmeng;HE Fumeng;FENG Xu;XU Yongqing;JIANG Xianfeng;WANG Xue;LIU Dan and LI Fenglan(College of Life Sciences,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030

出　　处：《西北农业学报》2023年第10期1594-1602,共9页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金青年项目(31201470);哈尔滨市应用科技研究与开发项目(2015RAQXJ021)。

摘　　要：旨在通过单因素试验对接骨木镰刀菌产呕吐毒素的温度、时间、pH、碳源及碳源浓度进行筛选,应用响应面法设计试验,依据Box-Behnken中心组合原理筛选得到影响接骨木镰刀菌产呕吐毒素的关键因素。以呕吐毒素含量为响应值借助Design-Expert v8.0.6软件分析各影响因子与响应值的相互作用,预测最适条件并验证。试验得到一个拟合程度高、误差小的模型,得出呕吐毒素含量最大时的条件为:葡萄糖为碳源、培养温度26.19℃、pH 6.12、培养时间25.38 d。在此条件下,呕吐毒素含量预测值为2.50 ng/mL,实际测得呕吐毒素含量为2.57 ng/mL。低浓度呕吐毒素处理能减小马铃薯病斑面积20%。In this study,we explored the impact of various factors on production of vomitoxin by Fusarium sambucinum,including temperature,time,pH,carbon sourceand carbon source concentration.To design the experiment,we employed response surface methodology,and screened the key factors affecting the production of vomitoxin by Fusarium sambucinum using the Box-Behnken center combination principle.The response value of vomitoxin content was analyzed using Design-Expert v8.0.6 software to determine the optimum conditions and verify it.By experimenting with the interaction of each influencing factor on the response value,a model with high fit and low error was obtained.The conditions for obtaining maximal vomitoxin content was determined to be glucose as carbon source,an incubation temperature of 26.19℃,pH 6.12,and an incubation time of 25.38 d.Under these conditions,the predicted vomitoxin content was 2.50 ng/mL,and the actual vomitoxin content was 2.57 ng/ml,treatment with low concentrations of vomitoxin reduced potato lesion area by 20%.

关 键 词：马铃薯 干腐病 镰刀菌 响应面法 呕吐毒素 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]