Subinhibitory Levels of Fluoroquinolones Result in Enrichment of the Membrane Proteome of Staphylococcus aureus  

作　　者：XU Xiaoxiao LIN Hong GUO Jiamin LIU Pei SUN Haixin 

机构地区：[1]College of Life Sciences,Qingdao University,Qingdao 266071,China [2]Food Safety Laboratory,College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China [3]Institute of Biomedical Engineering,Qingdao University,Qingdao 266071,China

出　　处：《Journal of Ocean University of China》2023年第5期1439-1445,共7页中国海洋大学学报（英文版）

基　　金：funded by the Professional Innovation and Integration Project of Qingdao University(2020)。

摘　　要：Staphylococcus aureus is a common marine foodborne pathogen.In this study,antibiotics ciprofloxacin and enrofloxacin were used to induce drug-resistance in S.aureus.The differentially expressed proteins(DEPs)were analyzed and compared with those in the bacteria cultured without antibiotics.The primary proteomic alterations were in the levels of cell membrane components and proteins related to lysine and folic acid biosynthesis,which were all significantly up-regulated.The minimal inhibitory concentrations(MIC)for both test drugs were elevated to 10μg m L^(-1)following serial passaging.These results indicated that,for both ciprofloxacin and enrofloxacin,drug-resistance were developed even in the subinhibitory levels and the primary response was a major alteration in the cell membrane proteome.These changes were similar to those observed in S.aureus cultured with super-MIC levels of these antibiotics.The current study provides a theoretical basis for in-depth study of the related changes of marine foodborne pathogens in subinhibitory concentrations that are commonly found in situ.

关 键 词：subinhibitory environment marine foodborne pathogen PROTEOMICS FLUOROQUINOLONE 

分 类 号：X56[环境科学与工程—环境工程]