表没食子儿茶素没食子酸酯通过PI3K/Akt通路抑制胰腺癌细胞糖酵解的实验研究  

EGCG inhibits glycolysis of pancreatic cancer cells via PI3K/AKT pathway

作　　者：高静[1] 王丰[2] 胡立娟 GAO Jing;WANG Feng;HU Li-juan(The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin(300000),China)

机构地区：[1]天津中医药大学第一附属医院,国家中医针灸临床医学研究中心,天津300000 [2]天津市中西医结合医院(天津市南开医院),天津300100

出　　处：《中国中西医结合外科杂志》2023年第5期677-681,共5页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：天津市教育委员会自然科学类一般项目(2022KJ269)。

摘　　要：目的:探讨表没食子儿茶素没食子酸酯(EGCG)对胰腺癌细胞的增殖及糖代谢的影响。方法:用CCK8试剂盒检测不同浓度EGCG对胰腺癌PANC-1细胞增殖的影响,用葡萄糖乳酸试剂盒检测EGCG对糖酵解的影响,Western blot法检测糖酵解酶及PI3K/Akt通路蛋白的表达。结果:CCK8结果显示,EGCG可以抑制PANC-1细胞的增殖,其抑制作用具有剂量依赖性和时间依赖性(P<0.05)。葡萄糖乳酸检测实验表明,EGCG可以抑制PANC-1细胞的葡萄糖消耗和乳酸生成(P<0.05)。Western blot结果表明,EGCG可以抑制糖酵解酶己糖激酶-2(HK-2)、磷酸果糖激酶(PFK)和乳酸脱氢酶(LDH)及磷酸化PI3K和Akt的表达,且EGCG浓度越高,抑制作用越明显(P<0.05)。结论:EGCG抑制人胰腺癌细胞系PANC-1的增殖和糖酵解,其抑制作用可能与抑制PANC-1细胞中PI3K/Akt通路有关。Objective To investigate the effects of epigallocatechin gallate(EGCG)on the proliferation and glucose metabolism of pancreatic cancer cells.Methods Human pancreatic cancer PANC-1 cells were treated with different concentrations of EGCG,and the proliferation of PANC-1 cells was assessed by CCK8 method.The effects of EGCG on PANC-1 cell glycolysis were detected by glucose consumption and lactate production,and the expressions of glycolytic enzyme and PI3K/Akt pathway protein were detected by Western blot.Results The results of CCK8 assay showed that EGCG inhibited the proliferation of pancreatic cancer PANC-1 cells in a dose-and time-dependent manner(P 0.05).EGCG also inhibited the glucose consumption and lactate production of PANC-1 cells(P 0.05).Compared with the control group,EGCG inhibited the expression of glycolytic enzyme and PI3K/Akt pathway protein in a dose-dependent manner(P 0.05).Conclusion EGCG can inhibit the proliferation and glycolysis of human pancreatic cancer cells,which may be related to the inhibition of PI3K/Akt pathway in PANC-1 cells.

关 键 词：胰腺癌 表没食子儿茶素没食子酸酯 糖酵解 细胞增殖 PI3K/AKT通路 

分 类 号：R735.9[医药卫生—肿瘤]