欧李种质资源果实酚类物质分析及种质评价  

Fruit Phenolic Content Analysis and Germplasm Evaluation of Cerasus humilis Germplasm Resources

作　　者：邵郅胜 张薇 孙聪 郭金丽[1] SHAO Zhisheng;ZHANG Wei;SUN Cong;GUO Jinli(College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010018,China)

机构地区：[1]内蒙古农业大学园艺与植物保护学院,呼和浩特010018

出　　处：《西北植物学报》2023年第8期1405-1414,共10页Acta Botanica Boreali-Occidentalia Sinica

基　　金：内蒙古科技成果转化专项(2019CG067);内蒙古自治区直属高校科技领军人才和创新团队建设专项(BR22-11-11);品种选育专项(YZGC2017019)。

摘　　要：以内蒙古地区36种欧李优系资源果实为试验材料,分析欧李果实品质性状的9项指标含量差异及变异系数探究其多样性,通过相关性、主成分、聚类分析对其品质性状进行综合评价,筛选酚类品质优异的欧李优系资源,为内蒙古欧李种质资源创新利用提供理论依据。结果表明,(1)9项品质性状指标变异系数为5.91%~31.03%,总黄酮最高,可滴定酸最低。(2)欧李果实3种酚类物质与总还原力、·OH清除率、DPPH·清除率及果肉色泽多表现极显著正相关(P<0.01)。(3)通过主成分分析提取的3个主成分累计贡献率为73.54%,分别为酚类因子、酸度因子和总抗氧化因子;建立欧李种质综合评价数学模型:F=[10×(46.213×F_(1)+15.132×F_(2)+12.197×F_(3))/73.542]+60,综合得分最高为MY37(84.53),综合表现最差为MY9(29.61)。(4)系统聚类评价,将36份优系资源聚为4大类,其中第Ⅰ类群综合表现最优,分别为MY5、MY36、MY37、MY38、MY41、MY47、MY50,为酚类物质积累型,多为红色果肉,酸含量适中,同时这7份优系资源综合得分均在前10位,此类群可作为高酚类品质欧李种质资源。In this study,36 Cerasus humilis resources in Inner Mongolia were selected as experimental materials,and the content differences and coefficient of variation of 9 indexes of C.humilis fruit quality traits were analyzed for exploring their diversity.The quality traits were comprehensively evaluated through correlation,principal component and cluster analysis,and the C.humilis superior resources with excellent phenolic quality were screened.This study provided theoretical basis for the innovative utilization of germplasm resources of C.humilis in Inner Mongolia.The results show that:(1)The variation coefficients of 9 quality traits ranged from 5.91%to 31.03%,the highest was total flavone,and the lowest was titratable acid.(2)The three phenolic contents in C.humilis fruits were significantly positively correlated with total reducing power,·OH clearance rate,DPPH·clearance rate and flesh color(P<0.01).(3)The cumulative contribution rate of the three principal components extracted by principal component analysis was 73.54%,which were phenolic factors,acidity factors and total antioxidant factors.The mathematical model for comprehensive evaluation of C.humilis germplasm was established as follows:F=[10×(46.213×F_(1)+15.132×F_(2)+12.197×F_(3))/73.542]+60,the highest comprehensive score was MY37(84.53),and the worst comprehensive performance was MY9(29.61).(4)In the systematic cluster evaluation,the 36 C.humilis superior resources were grouped into four categories,among which groupⅠhad the best comprehensive performance,which were MY5,MY36,MY37,MY38,MY41,MY47 and MY50,which were the accumulation type of phenolic content,mostly red fruit meat and moderate acid content.Meanwhile,the comprehensive scores of these 7 superior resources were all among the top 10.This group can continue to breed new varieties of C.humilis with high phenolic quality.

关 键 词：欧李 果实 酚类物质 种质评价 

分 类 号：S662.5[农业科学—果树学]