产褐藻胶裂解酶菌株的筛选与鉴定  

Screening and Identification of Alginate Lyase-Producing Strains

作　　者：李梦 温少红[1] 王坤阳 李莉莉[2,3] 焦绪栋[2,3] LI Meng;WEN Shaohong;WANG Kunyang;LI Lili;JIAO Xudong(College of Life Science,Yantai University,Yantai 264005,China;Yantai Coastal Zone Institute,Chinese Academy of Sciences,Yantai 264003,China;Center for Ocean Mega Science,Chinese Academy of Sciences,Qingdao 266071,China)

机构地区：[1]烟台大学生命科学学院,山东烟台264005 [2]中国科学院烟台海岸带研究所,山东烟台264003 [3]中国科学院海洋大科学研究中心,山东青岛266071

出　　处：《食品科技》2023年第7期1-9,共9页Food Science and Technology

基　　金：烟台市科技计划项目(2021XDHZ059)。

摘　　要：目的:筛选产生褐藻胶裂解酶的菌株,提高褐藻胶裂解酶的生产水平,为褐藻胶生物酶工业化生产和应用提供条件。方法:以海藻酸钠为唯一碳源,从烟台海岸带土壤中筛选菌株,通过形态学观察、生理生化分析和16S rDNA序列比对进行菌种鉴定,优化其产酶条件,分析酶解产物构成。结果:筛选得一株高产褐藻胶裂解酶的类芽孢杆菌(Paenibacillus)PLT1;最优产酶条件为:海藻酸钠9.5 g/L、蛋白胨6.7 g/L、Na Cl 0.5 g/L、接种量2%、培养温度31.0℃、摇床转速170 r/min、培养基初始p H值为7时,酶活可达214.64 U/mL。该酶降解海藻酸钠的酶解产物为三糖、四糖和五糖。结论:筛选到褐藻胶裂解酶高效产酶菌株Paenibacillus sp.PLT1,可用于发酵生产褐藻胶裂解酶或褐藻寡糖,在食品加工领域具有较高的应用价值。Objective:To screen the strains producing alginate lyase,improve the production level of alginate lyase,and provide conditions for industrial production and application of alginate lyase.Methods:With sodium alginate as the sole carbon source,the strain was screened from the soil of Yantai coastal zone.The strain was identified by morphological observation,physiological and biochemical analysis and 16S rDNA sequence alignment.The enzyme production conditions were optimized and the composition of enzymatic hydrolysate was analyzed.Result:A strain of Paenibacillus PLT1 with high yield of alginate lyase was screened.The optimal enzyme production conditions were as follows:sodium alginate 9.5 g/L,peptone 6.7 g/L,NaCl 0.5 g/L,inoculation amount 2%,culture temperature 31.0℃,shaking speed 170 r/min,and initial pH of medium 7.The enzyme activity could reach 214.64 U/mL.The enzymatic degradation products of sodium alginate were trisaccharide,tetrasaccharide and pentasaccharide.Conclusion:The strain Paenibacillus sp.PLT1 with high alginate lyase production efficiency was screened,which could be used to produce alginate lyase or alginate oligosaccharide by fermentation,and had high application value in the field of food processing.

关 键 词：褐藻胶裂解酶 筛选 鉴定 褐藻胶寡糖 

分 类 号：TS201.3[轻工技术与工程—食品科学]