蒙古扁桃叶绿体基因组比较  

Comparison of Chloroplast Genome of Amygdalus mongolica

作　　者：段春燕[1] 段义忠[2] DUAN Chunyan;DUAN Yizhong(College of Agriculture/Peony College,He'nan University of Science and Technology,Luoyang He'nan 471023,China;College of Life Sciences,Yulin University,Yulin Shaanxi 719000,China)

机构地区：[1]河南科技大学农学院/牡丹学院,河南洛阳471023 [2]榆林学院生命科学学院,陕西榆林719000

出　　处：《种子》2023年第8期5-10,F0002,共7页Seed

基　　金：国家自然科学基金(32060095);河南省特色骨干学科建设——旱地绿色智慧农业学科群(17100001);河南科技大学博士科研启动基金资助项目(4024-13480114)。

摘　　要：对濒危植物蒙古扁桃(Amygdalus mongolica)叶绿体基因组的序列特征进行对比分析,用QIAGEN试剂盒提取蒙古扁桃总DNA,采用Illumina HiSeq X^(TM)Ten平台建库测序,以KX255667为参考叶绿体基因序列,组装注释蒙古扁桃叶绿体基因序列取得NCBI注册号。研究发现,NCBI中3个蒙古扁桃叶绿体全基因序列长度为158039~158451 bp,序列长度差异主要在于LSC与IR。蒙古扁桃3个序列中具有相同数目的tRNA基因及rRNA基因,均有32个外显子。3个序列具有相似的密码子使用情况。本研究对蒙古扁桃叶绿体基因组进行结构研究并构建进化树,为研究桃类植物叶绿体基因工程和资源开发提供数据参考。Comparative analysis was conducted on the sequence characteristics of the chloroplast genome in the endangered plant Amygdalus mongolica.Total DNA of A.mongolica was extracted using QIAGEN kit and sequenced using Illumina Hiseq X TM Ten platform.Using KX255667 as the reference chloroplast gene sequence,the annotated chloroplast gene sequence of A.mongolica was assembled to obtain the NCBI registration number.The study showed that the sequence length of the three chloroplast genes of A.mongolica in NCBI ranged from 158039 bp to 158451 bp,and the difference of sequence length was mainly in LSC and IR.There were the same number of tRNA genes and rRNA genes in the three sequences of A.mongolica,of which there were 32 exons in the cpDNA sequences.The three sequences had similar codon usage.In this study,the chloroplast genome structure of A.mongolica was studied based on the existing NCBI data and the evolutionary tree was constructed,which provided some data references for the chloroplast gene engineering and resource development of peach plants.

关 键 词：蒙古扁桃 叶绿体基因组 高通量测序 密码子 进化树 

分 类 号：S662.9[农业科学—果树学]