绣球菌浓缩液抑制黑色素合成和抗氧化活性研究  

Study on the inhibition of melanin synthesis and antioxidant activities of concentrated Sparassis crispa extract

作　　者：徐媛媛 陈萌 徐晓飞 何瑞琪 XU Yuan-Yuan;CHEN Meng;XU Xiao-Fei;HE Rui-Qi(College of Food Science and Engineering,Guangdong Ocean University,Yangjiang 529500,China;South China Institute of Collaborative Innovation,Dongguan 523808,China)

机构地区：[1]广东海洋大学食品科学与工程学院,阳江529500 [2]华南协同创新研究院,东莞523808

出　　处：《食品安全质量检测学报》2023年第17期261-268,共8页Journal of Food Safety and Quality

基　　金：广东海洋大学大学生创新创业项目。

摘　　要：目的探究绣球菌浓缩液(concentrated Sparassis crispa extract,SCE)对黑色素合成和氧化损伤成纤维细胞的影响。方法以酵母β-葡聚糖为对照,通过检测酪氨酸酶酶活抑制率、黑色素瘤B16细胞内黑色素产生、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除率及人成纤维细胞存活率、细胞内活性氧(reactive oxygen species,ROS)水平和细胞合成胶原蛋白、弹性蛋白的浓度,探讨SCE抑制黑色素合成和成纤维细胞氧化损伤的保护作用。结果在本研究的测试浓度范围内,SCE对酪氨酸酶活性抑制率为35.0%~79.4%,对黑色素瘤B16细胞黑色素产生抑制率为24.6%~52.1%,并表现出浓度依赖性。同时,SCE能有效清除DPPH自由基,对H_(2)O_(2)诱导的人成纤维细胞凋亡具有保护作用,并可降低细胞内ROS水平;与模型组比较,SCE在10~100μg/mL范围内对成纤维细胞合成Ⅲ型胶原蛋白提高了67%~79%、弹性蛋白合成提高了124%~199%,且效果优于酵母β-葡聚糖。结论SCE具有抑制黑色素合成、保护氧化损伤成纤维细胞和提高成纤维细胞合成胶原蛋白、弹性蛋白的作用,这为其应用于美容功能食品提供依据。Objective To explore the effects of the concentrated Sparassis crispa extract(SCE)on melanin synthesis and oxidatively damaged fibroblasts.Method Yeastβ-glucan was chosen as a control,the inhibition rate of tyrosinase activity,melanin synthesis in B16 melanoma cells,1,1-diphenyl-2-picrylhydrazyl(DPPH)radical clearance rate,human fibroblast survival rate,intracellular reactive oxygen species(ROS)levels,and the concentrations of collagen and elastin synthesized by fibroblasts were investigated to evaluate the inhibitory effect of SCE on melanin synthesis and its protective effect on oxidative damage of fibroblasts.Results The inhibition rates of tyrosinase activity of SCE were 35.0%-79.4%in the range of testing concentrations and the inhibition rates of melanin synthesis in B16 melanoma cells were 24.6%-52.1%,both exhibiting a concentration-dependent manner.Meanwhile,SCE could effectively scavenge DPPH radicals,have a protective effect on H_2O_2-induced apoptosis of human fibroblasts and reduce the levels of intracellular ROS.Furthermore,compared with the model,SCE increased the content of typeⅢcollagen by 67%-79%and elastin by 124%-199%in fibroblasts in the range of 10-100μg/mL.Particularly,the improvement effects of SCE were better than those of yeastβ-glucan regarding antioxidative actions.Conclusion SCE can inhibit melanin synthesis in B16 melanoma cells,protect fibroblasts from oxidative damages,and improve the synthesis of collagen and elastin in fibroblasts,which provides a basis for its application in cosmetic functional food.

关 键 词：绣球菌 黑色素 人成纤维细胞 胶原蛋白 弹性蛋白 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]