Regulation of FLC nuclear import by coordinated action of the NUP62-subcomplex and importinβSAD2  

作　　者：Gang Chen Danyun Xu Qing Liu Zhichuang Yue Biao Dai Shujuan Pan Yongqiang Chen Xinhua Feng Honghong Hu 

机构地区：[1]National Key Laboratory of Crop Genetic Improvement,Hubei Hongshan Laboratory,Huazhong Agricultural University,Wuhan 430070,China

出　　处：《Journal of Integrative Plant Biology》2023年第9期2086-2106,共21页植物学报（英文版）

基　　金：supported by grants from the National Natural Science Foundation(31970730 and 32170721)to H.H;the Baichuan felowship from College of Life Science and Technology,Huazhong Agricultural University to D.X。

摘　　要：Flowering locus C(FLC)is a central transcriptional repressor that controls flowering time.However,how FLC is imported into the nucleus is unknown.Here,we report that Arabidopsis nucleoporins 62(NUP62),NUP58,and NUP54 composed NUP62-subcomplex modulates FLC nuclear import during floral transition in an importinα-independent manner,via direct interaction.NUP62 recruits FLC to the cytoplasmic filaments and imports it into the nucleus through the NUP62-subcomplex composed central channel.Importinβsupersensitive to ABA and drought 2(SAD2),a carrier protein,is critical for FLC nuclear import and flower transition,which facilitates FLC import into the nucleus mainly through the NUP62-subcomplex.Proteomics,RNAseq,and cell biological analyses indicate that the NUP62-subcomplex mainly mediates the nuclear import of cargos with unconventional nuclear localization sequences(NLSs),such as FLC.Our findings illustrate the mechanisms of the NUP62-subcomplex and SAD2 on FLC nuclear import process and floral transition,and provide insights into the role of NUP62-subcomplex and SAD2 in protein nucleocytoplasmic transport in plants.

关 键 词：FLC flowering time IMPORTIN nuclear import NUCLEOPORIN 

分 类 号：Q943.2[生物学—植物学]