全血中24种挥发性有机物的顶空-气相色谱-质谱联用同时测定法  

Simultaneous determination of 24 volatile organic compounds in whole blood by headspace-gaschromatography-mass spectrometry

作　　者：何宗剑 朱定姬[1] 李燕[1] 周武旺[1] 覃利梅[1] 肖梅[1] HE Zongjian;ZHU Dingji;LI Yan;ZHOU Wuwang;QIN Limei;XIAO Mei(Department of Physical and Chemical Testing,Guangxi Zhuang Autonomous Region Academy for Prevention and Treatment of Occupational Diseases,Nanning Guangxi,530021,China)

机构地区：[1]广西壮族自治区职业病防治研究院理化检测所,广西南宁530021

出　　处：《职业与健康》2023年第17期2350-2353,共4页Occupation and Health

基　　金：广西壮族自治区卫生健康委员会课题(Z20211115)。

摘　　要：目的建立全血中24种挥发性有机物(volatile organic compounds,VOCs)的顶空-气相色谱-质谱联用(gas chromatography-mass spectrometry,GC-MS)测定方法。方法血样中VOCs经顶空进样器进样,色谱柱分离,GC-MS检测。结果该方法测定血中24种VOCs在10.0~3000.0 ng/mL范围内线性关系良好,相关系数均大于0.9994,方法检出限0.2~10.0 ng/mL,加标回收率在78.7%~116.4%之间,相对标准偏差为0.9%~8.3%,样品在4和-25℃下可保存3 d。结论该方法简单准确、灵敏高,适用于职业接触人员、中毒患者全血中挥发性有机物的检测。Objective To establish a method for the determination of 24 volatile organic compounds(VOCs)in whole blood by headspace-gas chromatography-mass spectrometry(GC-MS).Methods The VOCs in blood samples were separated by column after headspace injection,and detected by GC-MS.Results The linear relationship of 24 kinds of VOCs in blood was good in the range of 10.0-3000.0 ng/mL,the correlation coefficients were higher than 0.9994,the detection limit was 0.2-10.0 ng/mL,the recovery rate was 78.7%-116.4%,and the relative standard deviation was 0.9%-8.3%.The samples could be preserved at 4 or-25℃for three days.Conclusion This method is simple,accurate,and highly sensitive,which is suitable for the detection of volatile organic compound in whole blood of occupational exposure persons and poisoning patients.

关 键 词：顶空 气相色谱质谱联用 全血 挥发性有机物 

分 类 号：R115[医药卫生—公共卫生与预防医学]