脱脂南极磷虾蛋白质提取条件优化  

Optimization of extraction conditions for Defatted Antarctic krill protein

作　　者：耿乐 王洪斌 刘志东[2] 张俊杰 段蕊 林娜 倪玲 迟海[2] GENG Le;WANG Hongbin;LIU Zhidong;ZHANG Junjie;DUAN Rui;LIN Na;NI Ling;CHI Hai(School of Food Science and Engineering,Jiangsu Ocean University,Lianyungang,222005,Jiang Su China;East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China)

机构地区：[1]江苏海洋大学食品科学与工程学院,江苏连云港222005 [2]中国水产科学研究院东海水产研究所,上海200090

出　　处：《渔业现代化》2023年第5期71-78,共8页Fishery Modernization

基　　金：中国水产科学研究院基本科研业务费项目(2020XT1201)。

摘　　要：为探究脱脂南极磷虾蛋白质(Defatted Antarctic Krill Protein,DAKP)最佳提取条件,提高脱脂南极磷虾粉附加值。以脱脂南极磷虾粉为原料,采用碱溶酸沉法提取其蛋白质,以蛋白质得率为指标,对提取温度、提取时间、料液比、NaOH百分比浓度4个影响因素进行单因素试验并采用响应面法对提取工艺进行分析与优化。结果显示:通过响应面法优化得到DAKP实际最佳的提取条件为提取温度69℃、提取时间65 min、料液比1∶15、NaOH百分比浓度2.2%,通过验证试验得出此提取条件下实际蛋白质得率为31.93%±0.35%,与理论值32.32%相对误差为1.19%±1.01%。研究表明,该回归模型预测准确,可以用于指导生产实践,有利于提高脱脂南极磷虾粉附加值,推动南极磷虾产业的可持续发展。In order to explore the best extraction conditions of Defatted Antarctic Krill Protein(DAKP)and increase the added value of defatted Antarctic krill powder.Using defatted Antarctic krill powder as the raw material,and the protein was extracted by alkali solution and acid precipitation.Taking the protein yield as the index,the single factor test was carried out on the four influencing factors of extraction temperature,extraction time,solid-liquid ratio and NaOH concentration.The extraction process was analyzed and optimized by response surface methodology.The actual optimum extraction conditions of DAKP were optimized by response surface methodology as follows:extraction temperature 69℃,extraction time 65 min,solid-liquid ratio 1∶15,NaOH concentration 2.2%.Through validation test,the actual protein yield was 31.93%±0.35%,with a relative error of 1.19%±1.01%from the theoretical value of 32.32%.The results show that the regression model is accurate and can be used to guide the production practice,which is beneficial to improve the added value of the defatted Antarctic krill powder and promote the sustainable development of the Antarctic krill industry.

关 键 词：脱脂南极磷虾粉 蛋白质 碱溶酸沉法 响应面法 提取优化 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]