气相色谱-串联质谱法测定罗汉果中22种农药残留量  

Determination of 22 Pesticide Residues in Siraitia grosvenorii by Gas Chromatography Tandem Mass Spectrometry

作　　者：任永霞 黄纯婷 徐日文 翁福良 梁宁 REN Yongxia;HUANG Chunting;XU Riwen;WENG Fulang;LIANG Ning(Technical Center of Gongbei Customs District P.R.,Zhuhai 519000,China;Zhongshan PASSION Biotechnology Co.,Ltd.,Zhongshan 528400,China)

机构地区：[1]拱北海关技术中心,广东珠海519000 [2]中山百盛生物技术有限公司,广东中山528400

出　　处：《食品安全导刊》2023年第17期99-102,共4页China Food Safety Magazine

摘　　要：建立气相色谱-串联质谱法测定罗汉果中22种农药残留量的分析方法。样品用乙腈提取后经PSA/GCB固相萃取柱净化,采用气相色谱-串联质谱法检测,外标法定量。结果表明,所有农药在0.005~0.500μg·mL^(-1)线性关系良好,相关系数R2均大于0.999,方法定量限为0.010 mg·kg^(-1)。样品中3个加标水平的回收率为71.1%~116.7%,相对标准偏差为0.7%~7.5%。该方法准确并易于操作,符合农药残留检测要求,可对罗汉果中多种农药进行定性和定量检测。A method for the determination of 22 pesticide residues in Siraitia grosvenorii was established by gas chromatography tandem mass spectrometry.The sample was extracted with acetonitrile and purified by PSA/GCB solid-phase extraction column.Gas chromatography tandem mass spectrometry was used for detection,and external standard method was used for quantification.The results showed that all pesticides had good linear relationships within the range of 0.005~0.500μg·mL^(-1),with correlation coefficients R2 greater than 0.999.The quantitative limit of the method was 0.010 mg·kg^(-1).The recoveries of the three spiked levels in the sample were 71.1%~116.7%,with a relative standard deviation of 0.7%~7.5%.The method is accurate and easy to operate,and meets the requirements of pesticide residue detection.It can be used for qualitative and quantitative detection of various pesticides in Siraitia grosvenorii.

关 键 词：罗汉果 气相色谱-串联质谱 固相萃取 

分 类 号：O657.63[理学—分析化学] TS255.7[理学—化学]