虾壳活性肽对斑马鱼氧化应激损伤的保护作用  

Protective effects of Procambarus clarkii shell bioactive peptides on oxidative stress injury of zebrafish(Danio rerio)

作　　者：贺舒雯 朱豪杰 韩鹏薇 杨赛杰 崔波 程云辉[1] 李虹辉 文李[1] HE Shuwen;ZHU Haojie;HAN Pengwei;YANG Saijie;CUI Bo;CHENG Yunhui;LI Honghui;WEN Li(School of Food Science and Bioengineering,Changsha University of Science&Technology,Changsha,Hunan 410114,China;School of Food Science and Engineering,Qilu University of Technology,Jinan,Shandong 250353,China)

机构地区：[1]长沙理工大学食品与生物工程学院,湖南长沙410114 [2]齐鲁工业大学食品科学与工程学院,山东济南250353

出　　处：《食品与机械》2023年第9期140-147,共8页Food and Machinery

基　　金：湖南省自然科学基金青年项目(编号:2021JJ40567);国家自然科学基金青年项目(编号:32102816);国家自然科学基金面上项目(编号:31972077)。

摘　　要：目的:探究虾壳活性肽(PCSBP)对机体氧化应激损伤的保护作用。方法:采用碱性蛋白酶水解制备虾壳活性肽,测定其体外抗氧化活性,并分析PCSBP对禁食胁迫下斑马鱼氧化应激损伤的保护作用。结果:PCSBP在体外表现出潜在的抗氧化能力,其对DPPH自由基和羟自由基的清除率分别为(46.35±1.32)%和(75.22±2.18)%。禁食胁迫下斑马鱼的终末体重、体长和肌肉氨基酸含量均有所降低,而摄入PCSBP后能有效提高其生长性能和肌肉氨基酸含量;肌肉组织结构显示,禁食后斑马鱼肌纤维间隙增宽,出现断裂现象,摄入PCSBP后肌纤维排列整齐,形态结构完整,未见明显断裂;在抗氧化应激方面,PCSBP显著增强了斑马鱼肌肉超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)活性和谷胱甘肽(GSH)含量,同时还减少了氧化损伤指标丙二醛(MDA)和蛋白质羰基(PC)的生成量;进一步分析发现,PCSBP显著上调抗氧化信号分子nrf2基因表达,下调keap1表达,并增加其下游抗氧化酶基因转录表达,有效改善了斑马鱼肌肉氧化应激损伤。结论:虾壳活性肽对斑马鱼的氧化应激损伤有一定保护作用,可作为潜在的抗氧化剂。Objective:Explore the protective effect of Procambarus clarkii shell bioactive peptides(PCSBP)on oxidative stress injury in the organism.Methods:This study using alkaline protease hydrolysis method to prepare bioactive peptides and measured their antioxidant activity in vitro.Subsequently,the protective effects of PCSBP on oxidative stress injury in zebrafish under fasting stress was analyzed.Results:The PCSBP showed potential antioxidant capacity in vitro,with scavenging rates of DPPH radicals and hydroxyl radicals at(46.35±1.32)%and(75.22±2.18)%.The final body weight,body length and muscle amino acid content of zebrafish were decreased under fasting stress,but the growth performance and muscle amino acid content of Zebrafish were effectively improved after taking PCSBP.The muscle tissue structure showed that the myofiber gap widened and ruptured in zebrafish after fasting.The myofiber were arranged neatly,with complete morphology and structure after PCSBP ingestion,and no obvious rupture was observed.In terms of anti-oxidative stress,PCSBP significantly enhanced the activities of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-PX),and glutathione(GSH)content in zebrafish muscle,and also decreased the production of malondialdehyde(MDA)and protein carbonyl(PC),which were oxidative damage indexes.Further analysis revealed that PCSBP significantly up-regulated the expression of antioxidant signaling molecule nrf2 mRNA,down-regulated keap 1 expression,and increased the transcriptional expression of downstream antioxidant enzyme genes,which effectively ameliorated the oxidative stress damage in muscle of zebrafish.Conclusion:Bioactive peptides derived from crayfish shell has a protective effect on oxidative stress injury in zebrafish and can be used as a potential antioxidant.

关 键 词：克氏原螯虾壳 生物活性肽 斑马鱼 氧化应激 保护作用 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]