轮叶党参多糖的分离纯化及结构研究  

Isolation,purification and structure analysis of polysaccharide from lance asiabell root

作　　者：刘艳秋[1] 王亚茹 毛迪锐[1] 常凯[1] LIU Yanqiu;WANG Yaru;MAO Dirui;CHANG Kai(Forestry College of Beihua University,Jilin,Jilin 132013,China)

机构地区：[1]北华大学林学院,吉林吉林132013

出　　处：《食品与机械》2023年第9期162-168,240,共8页Food and Machinery

基　　金：吉林省自然科学基金(面上项目)(编号:20230101246JC);吉林省教育厅科学研究项目(编号:JJKH20230076KJ)。

摘　　要：目的:确定轮叶党参多糖组分1(CLPS1)结构,为轮叶党参多糖活性研究和天然植物多糖的开发提供依据。方法:经提取、分离纯化后获得组分CLPS1,利用气相色谱、红外光谱、核磁共振、高碘酸氧化、Smith降解和甲基化分析方法等确定CLPS1结构。结果:CLPS1比旋光度为右旋44°,总糖含量为97.6%,糖醛酸为13.18%;相对分子质量为91.7,CLPS1单糖组成为阿拉伯糖、半乳糖、葡萄糖、半乳糖醛酸、甘露糖,摩尔比为2.7∶3.4∶2.3∶1.1∶0.5。CLPS1中存在葡萄糖醛酸,分子中含有吡喃糖并且存在α-和β-构型。主链主要由阿拉伯糖和半乳糖组成,半乳糖和葡萄糖构成支链和主链的末端残基;半乳糖分支点糖残基是葡萄糖(1→4,6)、半乳糖(1→3,6);半乳糖有1→6、1→3,6、1→、1→3、1→4键型;葡萄糖有1→、1→4、1→4,6键型;阿拉伯糖为1→5键型,半乳糖醛酸为1→3键型。结论:CLPS1为一种结构复杂的酸性多糖。Objective:The structure of CLPS1(polysaccharide fraction 1 of Codonopsis lanceolata)was determined to provide a basis for the study of polysaccharide activity of C.lanceolata and the development of natural plant polysaccharides.Methods:After extraction,separation,and purification,the separation component CLPS1 was determined by gas chromatography,infrared spectroscopy,nuclear magnetic resonance,periodate oxidation,Smith degradation,and methylation analysis to determine the structure of CLPS1.Results:The specific rotation of CLPS1 is+44°,the content of the total polysaccharide is 97.6%,the uronic acid of CLPS1 is 13.18%,and the molecular weight of CLPS1 is 91.7.The monosaccharide component of CLPS1 contains Ara,Gal,Glc,GalA,The molar ratio is 2.7∶3.4∶2.3∶1.1∶0.5.Chemical composition and structure of polysaccharide CLPS1 was studied by infrared spectrum,NMR,partial hydrolysis with acid degradation,Smith,methylation and GC-MS.The results showed that monosaccharide compositions of CLPS1 contained GluA and pyranose,while pyranose included bothα-pyranoside andβ-pyranoside in structure.The backbone was composed of Ara and Gal,the brancd with Gal and the terminal residues of backbone with Glu.Branch point of Gal residues is linked by Glc(1→4,6);Gal(1→3,6);Gal contains 1→,1→3,1→4,1→6,1→3,6;Gal contains 1→,1→3,1→4,1→6,1→3,6;Glc contains 1→,1→4,1→4,6;Ara contains 1→5;Galacturonic acid 1→3.Conclusion:CLPS1 is a complex acidic polysaccharide.The structure analysis of C.lanceolata polysaccharides is the basis for studying the structure-activity relationship of C.lanceolata polysaccharides,which is of great significance for understanding the biological activity and mechanism of C.lanceolata polysaccharides.

关 键 词：轮叶党参 多糖 水提醇沉 柱层析 结构特征 

分 类 号：TS201.2[轻工技术与工程—食品科学]