大孔树脂纯化艾草多酚及其对α-葡萄糖苷酶活性抑制作用  

Purification of polyphenols from wormwood by macroporous resin and inhibitory activity of its againstα-glucosidase

作　　者：吴萍香 魏明[1] 丁凯莉 聂风 WU Ping-xiang;WEI Ming;DING Kai-li;NIE Feng(School of Biological and Food Engineering,Anhui Polytechnic University,Wuhu,Anhui 241000,China)

机构地区：[1]安徽工程大学生物与食品工程学院,安徽芜湖241000

出　　处：《食品与机械》2023年第7期13-19,共7页Food and Machinery

基　　金：安徽省科技重大专项(编号:202203a06020029);企业委托项目(编号:HX-2021-12-163)。

摘　　要：目的:分离纯化艾草多酚,并研究其对α-葡萄糖苷酶的抑制活性。方法:对比了7种大孔树脂对艾草多酚的吸附与解吸性能的差异,得到较合适的树脂,优化了其纯化条件。采用乙醇溶液进行梯度洗脱,研究了不同梯度乙醇洗脱组分对α-葡萄糖苷酶活性的抑制作用。结果:D101树脂是较好的艾草多酚吸附剂,静态吸附120 min即达到饱和,最佳分离纯化条件为:粗多酚质量浓度1.5 mg/mL,pH 2.0,上样流速1.5 mL/min,吸附平衡后,以60%乙醇为洗脱液,1.5 mL/min流速进行洗脱,纯化效果良好,多酚纯度由21.42%提升到69.19%,纯度提高了3.23倍。对比不同梯度乙醇洗脱组分对α-葡萄糖苷酶抑制活性,其中60%乙醇洗脱组分可以很好地抑制α-葡萄糖苷酶活性。60%乙醇洗脱的艾草多酚主要含有异槲皮苷、根皮苷、芦丁等,其可能对α-葡萄糖苷酶活性具有抑制作用。结论:艾草多酚具有一定的降血糖效果。Objective:This study aimed to purify crude polyphenols from wormwood and to study inhibitory activity of its againstα-glucosidase.Methods:The adsorption-desorption properties of wormwood polyphenols by seven kinds of macroporous resins were studied.The suitable resin for separating and purifying Wormwood polyphenols was obtained and the purification parameters were optimized.Using different concentration ethanol solution for dynamic elution,the inhibitory activity of different elution fractions onα-glucosidase was investigated.Results:The results indicated that D101 resin was a suitable material for purifying wormwood polyphenols and the macroporous resin reached equilibrium within 120 min.The optimal conditions for purifying wormwood polyphenols were as follows,crude polyphenol 1.5 mg/mL,pH 2.0,flow velocity of 1.5 mL/min,and 60%ethanol was used as the eluent with the elution flow rate of 1.5 mL/min.The purity of polyphenols increased from 21.42%to 69.19%after purification by D101 resin,which was enhanced 3.23 times.Different elution components were obtained by using different concentration ethanol as eluents,respectively.The 60%ethanol elution fraction that contained isoquercitrin,phloridzin and rutin had the best inhibitory effect onα-glucosidase activity.Conclusion:wormwood polyphenols have good development prospects for hypoglycemic effect.

关 键 词：艾草 多酚 大孔树脂 纯化 Α-葡萄糖苷酶活性 

分 类 号：TQ28[化学工程—有机化工]