超高效液相色谱-串联质谱法同时检测肉制品中17种合成色素的含量  

Determination of 17 Kinds of Synthetic Colorants in Meat Products by UPLC-MS/MS

作　　者：龚蕾 韩智 彭青枝 周陶鸿 黄徽 于果 王福燕[4] GONG Lei;HAN Zhi;PENG Qingzhi;ZHOU Taohong;HUANG Hui;YU Guo;WANG Fuyan(Hubei Provincial Institute for Food Supervision and Test,Wuhan 430075,China;Key Laboratory of Detection Technology of Focus Chemical Hazards in Animal-derived Food for State Market Regulation,Wuhan 430075,China;Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test,Wuhan 430075,China;School of Medicine,Jianghan University,Wuhan 430056,China)

机构地区：[1]湖北省食品质量安全监督检验研究院,湖北武汉430075 [2]国家市场监管重点实验室(动物源性食品中重点化学危害物检测技术),湖北武汉430075 [3]湖北省食品质量安全检测工程技术研究中心,湖北武汉430075 [4]江汉大学医学部,湖北武汉430056

出　　处：《食品工业科技》2023年第21期309-315,共7页Science and Technology of Food Industry

基　　金：湖北省市场监督管理局科技计划项目(Hbscjg-JS2021009);湖北省重点研发计划项目(2020BCA091)。

摘　　要：为了建立超高效液相色谱-串联质谱法(UPLC-MS/MS)同时快速检测肉制品中17种合成色素的方法。经水振荡提取样品,正己烷除油,弱阴离子固相萃取柱(WAX)富集净化,以乙腈-5 mmol/L乙酸铵为流动相进行梯度洗脱,采取负离子扫描,在多反应监测模式(MRM)下,使17种目标化合物在ACQUITY UPLC HSS T3反相色谱柱上分离,使用外标法定量。结果表明,17种目标化合物在20~1200μg/L的线性范围内相关系数均大于0.99,方法的检出限为0.01~0.05 mg/kg,定量限为0.03~0.15 mg/kg。17种目标化合物在阴性基质中通过添加3个不同加标水平(0.15、0.30和1.0 mg/kg)进行回收率测试,不同水平下的回收率为71.2%~103.5%,相对标准偏差(RSDs,n=6)范围为1.3%~4.9%。该方法净化效果好、操作简单、定量准确,适用于肉制品中合成色素的快速筛查以及快速定量分析。To establish a method for the rapid detection of 17 colorants in meat products by ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS),samples were homogeneously extracted with water,degreased with n-hexane,enriched and purified with weak anion solid phase extraction(WAX)column.The seventeen compounds were separated on ACQUITY UPLC HSS T3 column by the gradient elution using acetonitrile-5 mmol/L ammonium acetate solution as mobile phase,detected by MS/MS with negative mode in multiple reaction monitoring(MRM)mode,and finally quantified by external standard method.The correlation coeffcients(r)of the seventeen compounds were above 0.99 in the mass concentration ranging from 20~1200μg/L.The limits of detection and the limits of quantification of the method were 0.01~0.05 mg/kg and 0.03~0.15 mg/kg,respectively.The recoveries of seventeen analyses spiked at three levels(0.15,0.30 and 1.0 mg/kg)in meat products ranged from 71.2%to 103.5%with the relative standard deviations(RSDs,n=6)ranged from 1.3%~4.9%.The method was easy to operate and with good purification effect.It could be applied to the rapid determination of the synthetic colorants in meat products.

关 键 词：超高效液相色谱-串联质谱 色素 肉制品 弱阴离子固相萃取柱 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]