紫色红曲霉Mrr2基因的过表达对桔霉素积累的影响  

Effect of Overexpression of Mrr2 Gene in Monascus purpureus on Citrinin Accumulation

作　　者：唐光甫 满海乔 赵杰宏 韩洁 TANG Guang-fu;MAN Hai-qiao;ZHAO Jie-hong(Guizhou University of Traditional Chinese Medicine/Key Lab of Pharmacognostics of Guizhou Province,Guiyang,Guizhou 550025)

机构地区：[1]贵州中医药大学/贵州省中药生药学重点实验室,贵州贵阳550025

出　　处：《安徽农业科学》2023年第20期1-5,共5页Journal of Anhui Agricultural Sciences

基　　金：贵州省科技支撑项目(黔科合支撑[2019]2776号);国家自然科学基金项目(81960692)。

摘　　要：为研究红曲霉Mrr2基因对桔霉素代谢的作用,通过农杆菌介导遗传转化获得Mrr2过表达菌株,与野生型菌株相比,Mrr2过表达导致菌丝中桔霉素含量显著增加,洛伐他汀和色素含量均有不同程度增加。RT-qPCR结果显示,Mrr2过表达对其他桔霉素相关基因的表达有明显促进作用,推测Mrr2对桔霉素的合成和积累具有重要影响,进一步丰富和完善了桔霉素合成调控途径,为桔霉素的消减提供理论依据。To study the effect of the Mrr2 gene on citrinin,the Mrr2 overexpressed strains were obtained by genetic transformation mediated by Agrobacterium.Compared with wild-type strains,the overexpression of Mrr2 significantly increased the content of citrinin in mycelia,the content of lovastatin and pigment increased in different degree.RT-qPCR results showed that the high expression of Mrr2 promotes significant change in other citrinin-related genes,suggesting that Mrr2 had important regulatory effect on citrinin synthesis,further enriching the regulatory genes of citrinin synthesis,and providing a theoretical basis for the reduction of citrinin.

关 键 词：紫色红曲霉 过表达 桔霉素 红曲色素 Mrr2基因 

分 类 号：Q93[生物学—微生物学]