糙皮侧耳PoEgt2基因克隆及不同发酵时期表达水平分析  

Cloning of PoEgt2 Gene from Pleurotus ostreatus and Analysis of Expression Levels at Different Fermentation Stages

作　　者：田缘 潘钰[1] 于冲[1] 夏海华[1] 胡基华[1] 毛雪 闫更轩 TIAN Yuan;PAN Yu;YU Chong(Institute of Microbiology,Heilongjiang Academy of Sciences,Harbin,Heilongjiang 150010)

机构地区：[1]黑龙江省科学院微生物研究所,黑龙江哈尔滨150010

出　　处：《安徽农业科学》2023年第20期114-117,共4页Journal of Anhui Agricultural Sciences

基　　金：黑龙江省科学院青年创新基金项目(CXMS2022SW01)。

摘　　要：从糙皮侧耳基因组中预测获得PoEgt2基因进行生物信息学分析和表达分析。结果显示:PoEgt2编码氨基酸546个,为胞内不稳定蛋白,无跨膜区和信号肽。蛋白质序列比对结果显示,糙皮侧耳PoEgt2与白黄侧耳、刺芹侧耳等侧耳属真菌同源性较高,与非侧耳属菌同源性较低。PoEgt2在糙皮侧耳发酵前期的表达量较高,于发酵4 d达到峰值,随后表达水平随时间下降。该研究可为高产麦角硫因的糙皮侧耳菌种改良和发酵条件优化提供科学依据。In this study,PoEgt2 was predicted from Pleurotus ostreatus genome for bioinformatics analysis and expression analysis.The results showed that PoEgt2 encoded 546 amino acids,which was an intracellular unstable protein without transmembrane region and signal peptide.The results of protein sequence alignment showed that PoEgt2 had high homology with P.cornucopiae and P.eryngii,but low homology with non-Pleuloplegia.The expression level of PoEgt2 was higher in the early stage of fermentation and reached its peak on the fourth day of fermentation,and then decreased with time.This study provided a scientific basis for the improvement of Pleurotus ostreatus strains with high ergothioneine yield and optimization of fermentation conditions.

关 键 词：麦角硫因 EGT2 糙皮侧耳 发酵 

分 类 号：S646.14[农业科学—蔬菜学]