功能核酸侧流层析传感器及其食品安全快速检测应用  

Functional nucleic acid lateral flow chromatography sensor and its rapid food safety detection application

作　　者：汪德颖 齐小花[1] 盛万里 裴佳欢 邹明强[1] WANG Deying;QI Xiaohua;SHENG Wanli;PEI Jiahuan;ZOU Mingqiang(Chinese Academy of Inspection and Quarantine&Key Laboratory of Food Quality and Safety for State Market Regulation,Beijing,100176,China;Department of Environment and Life,Beijing University of Technology;Technical Center of Hohhot Customs District)

机构地区：[1]中国检验检疫科学研究院&国家市场监管重点实验室(食品质量与安全),北京100176 [2]北京工业大学环境与生命学部 [3]呼和浩特海关技术中心

出　　处：《质量安全与检验检测》2023年第5期24-30,共7页QUALITY SAFETY INSPECTION AND TESTING

基　　金：海南省重点研发计划项目(ZDYF2021SHFZ069);国家重点研发计划项目(2022YFF0607903);中国检验检疫科学研究院基本科研业务费项目(2022JK18)。

摘　　要：食品消费的全球化及食源性疾病的增加,凸显了食品危害监测和食品安全评估的重要性,灵敏、便捷、低廉的生物传感快速检测技术成为食品安全研究热点。功能核酸(FNAs)由于具有高结合亲和力、特异性识别和催化活性、易于修饰等多种功能,被集成到侧向层析分析中构建简单且可持续的纸基层析生物传感器,以实现快速、无抗体、经济高效的靶标检测。本文主要介绍基于不同功能核酸的侧流层析传感器,包括基本原理、从样品预处理到信号转导的构建过程及在食品安全检测中的应用,对未来研究方向进行展望,以期为有效预防控制食品安全问题提供一种生物传感快速检测路径。The globalization of food consumption and the increase of foodborne diseases highlight the importance of food hazard monitoring and food safety assessment.Sensitive,convenient and cheap biosensing rapid detection technology has become a hotspot of food safety research.Functional nucleic acids(FNAs),due to their high binding affinity,specific recognition and catalytic activity,easy modification and other functions,are integrated into the lateral chromatography analysis to build a simple and sustainable paper base analysis biosensor,in order to achieve rapid,antibody-free,cost-efficient target detection.This paper mainly introduces based on different functions of nucleic acid lateral flow chromatography sensor,including the basic principle,from the sample pretreatment to the construction process of signal transduction and the application in food safety detection,for the future research direction,in order to effective prevention and control of food safety problems provide a biological sensing rapid detection pathway.

关 键 词：功能核酸 核酸侧流层析传感器 快速检测 食品安全 

分 类 号：TS207[轻工技术与工程—食品科学] TP212[轻工技术与工程—食品科学与工程]