LncRNA(HOTAIR)/miR-206/TAGLN2信号轴调控甲状腺乳头状癌细胞的侵袭  

LncRNA(HOTAIR)/miR-206/TAGLN2 signal axis regulates the invasion of papillarythyroid carcinoma cells

作　　者：侯丽杰 马承旭 王晶宇[3] 汤旭磊[1,2] 傅松波 HOU Lijie;MA Chengxu;WANG Jingyu;TANG Xulei;FU Songbo(The First Clinical School of Medicine,School of Basic Medicine,Lanzhou University,Lanzhou 730000;Department of Pathophysiology,School of Basic Medicine,Lanzhou University,Lanzhou 730000;Department of Endocrinology,the First Hospital of Lanzhou University,Lanzhou 730000,China)

机构地区：[1]兰州大学第一临床医学院,甘肃兰州730000 [2]兰州大学第一医院内分泌科,甘肃兰州730000 [3]兰州大学基础医学院病理生理教研室,甘肃兰州730000

出　　处：《基础医学与临床》2023年第11期1642-1647,共6页Basic and Clinical Medicine

基　　金：甘肃省自然科学基金(20JR10RA681,17JR5RA214);甘肃省内分泌疾病临床医学研究中心建设(20JR10FA667)。

摘　　要：目的探讨lncRNA(HOTAIR)/miR-206/TAGLN2信号轴调控人甲状腺乳头状癌(PTC)细胞系TPC-1侵袭的分子机制。方法收集兰州大学第一医院甲状腺乳头状癌组织和癌旁组织,用RT-qPCR和Western blot检测PTC组织和癌旁组织中HOTAIR、miR-206和TAGLN2的表达;构建miR-206过表达和HOTAIR低表达的TPC-1细胞。RT-qPCR和Western blot检测mRNA和蛋白的表达,Transwell小室法实验检测TPC-1细胞系的侵袭能力。结果与癌旁组织相比PTC组织中HOTAIR表达量增加2.48倍,miR-206-5p表达量降低至0.6,TAGLN2及其蛋白表达量增加(P<0.05);过表达miR-206后,TPC-1细胞中TAGLN2的蛋白表达量减少(P<0.05),且TPC-1细胞的侵袭能力受到了抑制。敲低HOTAIR后,TPC-1细胞中miR-206表达增加,TAGLN2表达降低,同时TPC-1细胞的侵袭能力也明显降低(P<0.05)。结论在甲状腺乳头状癌中,降低HOTAIR的表达水平,可促成miR-206的过表达,进一步抑制TAGLN2的表达,最终可降低甲状腺癌的侵袭能力。Objective To investigate the molecular mechanism of lncRNA(HOTAIR)/miR-206/TAGLN2 signaling axis in regulating the invasion of papillary thyroid carcinoma cells line TPC-1.Methods The papillary thyroid carcinoma(PTC)and paracancerous tissues were collected from the First Hospital of Lanzhou University,and the expression of lncRNA(HOTAIR),miR-206 and TAGLN2 in PTC tissues as well as paracancerous tissues was detected by RT-qPCR and Western blot;The thyroid papillary cancer cells with miR-206 over-expression and HOTAIR under-expression were constructed to detect the expression of target gene and its effect on the invasion of TPC-1 cells by Transwell assay.Results Compared with the adjacent tissue,the expression of HOTAIR in papilla-rythyroid cancer tissue was increased by 2.48 times.miR-206-5p was decreased down to 0.6,and the expression of TAGLN2 was increased(P<0.05).After over-expression of miR-206,the protein expression of TAGLN2 was decreased in TPC-1 cell,and the invasion of TPC-1 cells was inhibited(P<0.05).Knockdown of HOTAIR increased expression of miR-206 in TPC-1 cells and decreased the expression of TAGLN2.The Transwell assay showed that the invasion of TPC-1 cells was significantly reduced(P<0.05).Conclusions In PTC,reducing the expression of HOTAIR results in over-expression of miR-206 then further inhibits the expression of TAGLN2.The biological outcome is inhibition of invasion of thyroid cancer.

关 键 词：甲状腺乳头状癌 侵袭 HOTAIR miR-206 TAGLN2 

分 类 号：R736.1[医药卫生—肿瘤]